纳米Ag@Kanamycin复合材料的制备及抑菌性能研究  

Preparation and Bacteriostatic Properties of Ag@Kanamycin Nanocomposites

作　　者：陈惠惠 吴迎花 汪艳 胡瑞玲 吴苗 王嘉伟 郭少波 季晓晖[1,2] CHEN Hui-hui;WU Ying-hua;WANG Yan;HU Rui-ling;WU Miao;WANG Jia-wei;GUO Shao-bo;JI Xiao-hui(School of Chemistry and Environmental Science,Shaanxi University of Technology,Hanzhong 723000,China;Shaanxi Province Key Laboratory of Catalytic Foundation and Application,Hanzhong 723000,China)

机构地区：[1]陕西理工大学化学与环境科学学院,陕西汉中723000 [2]陕西省催化基础与应用重点实验室,陕西汉中723000

出　　处：《化学试剂》2024年第12期39-47,共9页Chemical Reagents

基　　金：秦巴生物资源与生态环境国家重点实验室科研基金项目(SXS-2105);陕西省自然科学基金项目(2023-JC-QN-0162)。

摘　　要：近年来,由于抗生素滥用导致耐药菌的出现,致使人类健康受到严重威胁。因此,迫切需要研发出一种新型、高效的抑菌剂。以三氟醋酸银(CF 3COOAg)为原材料,通过单液相化学还原法制备纳米银颗粒(Ag NPs),利用“浸渍法”结合市售抗生素硫酸卡那霉素(Kanamycin)合成了Ag@Kanamycin复合材料。通过TEM、XRD、UV-Vis、FT-IR、表面静电势(ESP)等对样品的形貌、晶体结构和键合方式等进行系统化表征。以大肠杆菌(E.coli)、金黄色葡萄球菌(S.aureus)和真菌白色念珠菌(C.albicans)为测试菌,通过最小抑菌浓度(MIC)、滤纸片扩散法、菌落计数法探究复合材料的协同抑菌性能和抑菌机制。抑菌活性显示,复合材料对3种测试菌的MIC分别为40、20和10μg/mL。抑菌机制表明,复合材料能够有效改变菌体细胞膜通透性并破坏细胞壁,导致细菌凋亡。In recent years,the overuse of antibiotics has led to the emergence of drug-resistant bacteria that pose a serious threat to human health.As a result,there is an urgent need to develop a new and effective bacteriostatic agent.Silver nanoparticles(Ag NPs)were prepared from silver trifluoroacetate(CF 3COOAg)by single-liquid-phase chemical reduction.The synthesis of Ag@Kanamycin composites was achieved using the"impregnation method"in combination with the commercially available antibiotic,kanamycin sulphate(Kanamycin).The samples were systematically characterized by TEM,XRD,UV-Vis,FT-IR and surface electrostatic potential(ESP)in terms of their morphology,crystal structure,and bonding mode.The filter paper diffusion method,colony counting method,and minimum inhibitory concentration(MIC)were employed to investigate the synergistic inhibitory properties and inhibitory mechanism of Ag@Kanamycin against Gram-negative Escherichia coli(E.coli),Gram-positive Staphylococcus aureus(S.aureus),and fungus Candida albicans(C.albicans).The inhibitory activity demonstrated that the MIC of the composites against the three tested bacteria was 40,20 and 10μg/mL,respectively.The mechanism of bacterial inhibition showed that the composites were effective in altering the permeability of the bacterial cell membrane and disrupting the cell wall,leading to bacterial apoptosis.

关 键 词：Ag NPs KANAMYCIN 协同抑菌 抑菌性能 抑菌机制 

分 类 号：TB333[一般工业技术—材料科学与工程]