白藜芦醇通过miR-23a靶向XIAP-caspase-3信号通路影响小鼠卵巢功能  

miR-23a targets the XIAP-caspase-3 signaling pathway to contribute to the effects of resveratrol on ovarian function in mice

作　　者：修银玲 于月新 孙凯旋 周静 赵盼盼 徐金龙 XIU Yinling;YU Yuexin;SUN Kaixuan;ZHOU Jing;ZHAO Panpan;XU Jinlong(Department of Reproductive Medicine,General Hospital of Northern Theater Command,Shenyang 110003,China;Pharmacy Department,The 969th Hospital of PLA Joint Logistic Support Force,Hohhot 010051,China)

机构地区：[1]中国人民解放军北部战区总医院生殖医学科,沈阳110003 [2]中国人民解放军联勤保障部队第九六九医院药剂科,呼和浩特010051

出　　处：《中国医科大学学报》2024年第11期1031-1035,共5页Journal of China Medical University

基　　金：辽宁省科学技术计划(2020JH2/10300118)。

摘　　要：目的观察白藜芦醇(RES)对小鼠卵巢功能的影响,探讨其中miR-23a的作用机制。方法通过腹腔注射环磷酰胺(120mg/kg)和丁磺芬(30 mg/kg),建立卵巢早衰(POF)小鼠模型。将30只小鼠随机分为对照组、模型组(POF组)和干预组(RES组),每组10只。测量小鼠体重、卵巢重量,计算卵巢指数;HE染色观察小鼠卵巢组织的病理变化;实时定量PCR检测卵巢组织中miR-23a、X连锁凋亡抑制蛋白(XIAP)和caspase-3 mRNA表达;Western blotting检测卵巢组织中XIAP和caspase-3蛋白表达。结果与对照组比较,POF组小鼠卵巢质量(P<0.05)、卵巢指数(P<0.05)和原始卵泡数量明显下降;与POF组比较,RES组小鼠卵巢质量(P<0.05)、卵巢指数(P<0.05)和原始卵泡数量明显增加。与对照组比较,POF组小鼠卵巢组织中miR-23a和caspase-3 mRNA表达水平升高,XIAP mRNA表达水平降低(均P<0.05);与POF组比较,RES组小鼠卵巢组织中miR-23a和caspase-3 mRNA表达水平降低,XIAP mRNA表达水平升高(均P<0.05)。与对照组比较,POF组小鼠卵巢组织中XIAP蛋白表达水平降低,caspase-3蛋白表达水平升高(均P<0.05);与POF组比较,RES组小鼠卵巢组织中XIAP蛋白表达水平升高,caspase-3蛋白表达水平降低(均P<0.05)。结论RES对卵巢功能减弱小鼠具有保护作用,其机制可能与RES影响miR-23a靶向XIAP-caspase-3信号通路有关。Objective To investigate the effect of resveratrol(RES)on ovarian function in mice and elucidate the potential mechanism involving miR-23a.Methods Thirty mice were randomly divided into control,premature ovarian failure(POF)model,and treatment(RES)groups,with n=10 per group.The body weight and ovarian mass of the mice were measured,and the ovarian index was calculated.Hematoxylin and eosin staining was performed to observe pathological changes in mouse ovarian tissue.Real-time quantitative PCR and Western blotting were performed to measure the mRNA and protein levels of miR-23a,X-linked inhibitor of apoptosis protein(XIAP),and caspase-3 in the ovarian tissue.Results Compared with the control group,the POF group exhibited significant decreases in ovarian mass(P<0.05),ovarian index(P<0.05),number of primary follicles,and XIAP mRNA expression(P<0.05),alongside significant increases in miR-23a and caspase-3 mRNA expression(P<0.05).Compared with the POF group,the RES group exhibited significant increases in the ovarian mass(P<0.05),ovarian index(P<0.05),number of primary follicles,and XIAP mRNA expression(P<0.05),as well as significant decreases in miR-23a and caspase-3 mRNA expression(P<0.05).XIAP protein expression was significantly lower and caspase-3 protein expression was significantly higher in the POF group than in the control group(P<0.05).Conversely,XIAP protein expression was significantly higher and caspase-3 protein expression was significantly lower in the RES group than in the POF group(P<0.05).Conclusion RES exerts a protective effect on weakened ovarian function in mice,potentially mediated through its effect on miR-23a targeting the XIAP-caspase-3 signaling pathway.

关 键 词：白藜芦醇 卵巢早衰 miR-23a X连锁凋亡抑制蛋白 CASPASE-3 

分 类 号：R711.75[医药卫生—妇产科学]