负载钛酸钙取向性静电纺聚己内酯/丝素蛋白支架促进周围神经再生  

Oriented electrostatically spun polycaprolactone/silk fibroin scaffold loaded with calcium titanate promotes peripheral nerve regeneration

作　　者：柳小军 商雨情 管文超 许琳琳 李贵才[3] Liu Xiaojun;Shang Yuqing;Guan Wenchao;Xu Linlin;Li Guicai(Henan Institute for Drug and Medical Device Inspection,Zhengzhou 450018,Henan Province,China;Key Laboratory for Quality Evaluation of Medical Protective and Implant Devices of National Medical Products Administration,Zhengzhou 450018,Henan Province,China;Key Laboratory of Neuroregeneration of Ministry of Education,Nantong University,Nantong 226001,Jiangsu Province,China)

机构地区：[1]河南省药品医疗器械检验院,河南省郑州市450018 [2]国家药监局医用生物防护及植入器械质量评价重点实验室,河南省郑州市450018 [3]南通大学神经再生教育部重点实验室,江苏省南通市226001

出　　处：《中国组织工程研究》2025年第28期6070-6082,共13页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(32171352),项目负责人:李贵才;国家药监局医用生物防护及植入器械质量评价重点实验室开放课题项目(2023-02),项目负责人:李贵才。

摘　　要：背景:取向性聚己内酯/丝素蛋白共混静电纺丝支架修复周围神经损伤可促进神经再生,但修复效果有限,还需要进一步结合生物活性材料、生长因子等因素制备仿生神经支架。钛酸钙纳米颗粒具有促进施万细胞间黏附的能力,已被广泛运用于生物医学材料领域。目的:采用静电纺丝技术制备取向性聚己内酯/丝素蛋白-钛酸钙功能化支架,进一步探索该支架对施万细胞活力与黏附的影响。方法:采用静电纺丝技术制备聚己内酯/丝素蛋白支架,通过调整制备过程中的注射器流速、接收装置转速、纺丝时间筛选最优参数进行后续实验。在最优参数下,采用静电纺丝技术制备随机取向聚己内酯/丝素蛋白支架(记为PCL/SF-R)、取向性聚己内酯/丝素蛋白支架(记为CTO-0)及含0.1,0.5,1.0 mg/mL聚多巴胺修饰钛酸钙的取向性聚己内酯/丝素蛋白支架(依次记为CTO-1、CTO-5、CTO-10),表征5组支架的物理性能。将5组支架浸提液分别与大鼠成纤维细胞系(L929)共培养,采用MTT法检测细胞增殖。将大鼠施万细胞系(RSC96)分别接种于5组支架上,进行细胞活力、细胞骨架染色及细胞骨架与细胞黏附相关基因检测。结果与结论:①制备静电纺丝支架的最优参数为:注射器流速15μL/min,接收装置转速1800 r/min,纺丝时间1 h;②PCL/SF-R组水接触角最大、弹性模量最小,CTO-0组水接触角最小、弹性模量最大;CTO-0组支架降解速率低于CTO-1组,并且随着取向性支架中钛酸钙质量浓度的增加,支架的降解速率降低;③5组支架对L929细胞无毒性作用,具有良好的生物相容性;CCK-8检测结果显示,CTO-10可提升RSC96细胞活力;细胞骨架染色结果显示,取向性支架上细胞的长宽比和细胞突起长度均高于随机取向支架;qPCR检测结果显示,CTO-5组、CTO-0组YAP、Cntn2 mRNA表达均高于PCL/SF-R组(P<0.05);④结果表明,取向性聚己内酯/丝素蛋白-钛酸钙支架具有�BACKGROUND:Oriented polycaprolactone/silk fibroin blend electrospinning scaffolds can repair peripheral nerve damage and promote nerve regeneration,but the repair effect is limited,and it is necessary to further combine bioactive materials,growth factors,and other factors to prepare bionic neural scaffolds.Calcium titanate nanoparticles have the ability to promote adhesion between Schwann cells and have been widely used in the field of biomedical materials.OBJECTIVE:To prepare polycaprolactone/silk fibroin-calcium titanate functionalized scaffolds by electrospinning technology,and to further explore the effect of the scaffold on Schwann cell viability and adhesion.METHODS:Polycaprolactone/silk fibroin scaffolds were prepared by electrospinning technology.The optimal parameters were selected by adjusting the syringe flow rate,receiving device speed and spinning time during the preparation process for subsequent experiments.Under the optimal parameters,randomly oriented polycaprolactone/silk fibroin scaffolds(denoted as PCL/SF-R),oriented polycaprolactone/silk fibroin scaffolds(denoted as CTO-0),and oriented polycaprolactone/silk fibroin scaffolds containing 0.1,0.5,and 1.0 mg/mL polydopamine modified calcium titanate(denoted as CTO-1,CTO-5,and CTO-10,respectively)were prepared by electrospinning technology.The physical properties of the five groups of scaffolds were characterized.The extracts of the five groups of scaffolds were co-cultured with rat fibroblast cell line(L929).Cell proliferation was detected by MTT assay.Rat Schwann cell line(RSC96)was inoculated on the five groups of scaffolds.Cell viability,cytoskeleton staining,and cytoskeleton and cell adhesion related genes were detected.RESULTS AND CONCLUSION:(1)The optimal parameters for preparing electrospinning scaffolds were as follows:syringe flow rate 15μL/min,receiving device speed 1800 r/min,and spinning time 1 hour.(2)The PCL/SF-R group had the largest water contact angle and the smallest elastic modulus,while the CTO-0 group had the smallest water

关 键 词：钛酸钙 取向性静电纺丝 聚己内酯 丝素蛋白 周围神经再生 工程化神经材料 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学] R745