基于酶功能化量子点的人尿液胰蛋白酶传感器  

Trypsin sensor in human urine based on enzyme-functionalized quantum dots

作　　者：雷少辉 郑冬云[1,2,3] 刘超 刘晓军 朱春楠[1,2,3] LEI Shaohui;ZHENG Dongyun;LIU Chao;LIU Xiaojun;ZHU Chunnan(College of Biomedical Engineering,SouthCentral Minzu University,Wuhan 430074,China;Hubei Key Laboratory of Medical Information Analysis and Tumor Diagnosis&Treatment,Wuhan 430074,China;Key Laboratory of Cognitive Science(State Ethnic Affairs Commission),Wuhan 430074,China)

机构地区：[1]中南民族大学生物医学工程学院,湖北武汉430074 [2]医学信息分析及肿瘤诊疗湖北省重点实验室,湖北武汉430074 [3]认知科学实验室(国家民委重点实验室),湖北武汉430074

出　　处：《传感器与微系统》2024年第12期100-104,共5页Transducer and Microsystem Technologies

基　　金：国家自然科学基金资助项目(21804146,22004134);湖北省自然科学基金资助项目(2023AFB799);中央高校基本科研业务费专项资金项目(CPT22022);横向项目(HZY22110)。

摘　　要：以葡萄糖氧化酶(GOD)为模板,制备了GOD功能化的锰掺杂硫化锌(GOD-Mn-ZnS)量子点(QDs),并通过正交实验对QDs的合成条件进行优化。构建了一种检测胰蛋白酶的磷光传感体系,实现了对人体尿液中胰蛋白酶的定量检测。实验结果表明,QDs的磷光猝灭程度与胰蛋白酶的浓度呈良好的线性关系,线性范围为5~100 mg/L,R^(2)=0.998,检出限为2.21 mg/L。采用标准加入法对健康志愿者的尿液进行检测,加标回收率为97.45%~103.00%。该方法可用于急性胰腺炎患者尿液中胰蛋白酶的快速检测,具有良好的生物医学应用前景。Glucose oxidase(GOD)-functionalized Mn-doped ZnS GOD-Mn-ZnS quantum dots(QDs),are prepared using GOD as the template.The synthesis conditions of QDs are optimized by orthogonal experiments.Then a phosphorescence sensing system for detecting trypsin is constructed,the quantitative detection of trypsin in human urine is realized.The phosphorescent quenching degree of QDs show a good linear relationship with the concentration of trypsin.The linear range is 5~100 mg/L,R^(2)=0.998,and the detection limit is 2.21 mg/L.The urine of healthy volunteers is detected by standard addition method,and the recovery rate is 97.45%~103.00%.The method can be used for rapid detection of trypsin in urine of patients with acute pancreatitis and has good biomedical application prospects.

关 键 词：量子点 酶功能化 胰蛋白酶检测 生物医学传感器 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置] O657.32[自动化与计算机技术—控制科学与工程]