同源卵母细胞在不同受精时间下的受精发育结局  

Effect of different fertilization time to fertilization and development outcomes of homologous oocytes

作　　者：王宇峰 于春梅 曹芳 戴秀亮 夏西洋 高亭亭 李玲君 WANG Yu-feng;YU Chun-mei;CAO Fang;DAI Xiu-liang;XIA Xi-yang;GAO Ting-ting;LI Ling-jun(Reproductive Medicine Center,Changzhou Maternal and Child Health Care Hospital,Changzhou,Jiangsu 213000,China)

机构地区：[1]常州市妇幼保健院生殖医学中心,江苏常州213000

出　　处：《医药论坛杂志》2024年第20期2147-2150,共4页Journal of Medical Forum

摘　　要：目的研究短时及长时受精对同源卵母细胞受精和胚胎发育产生的影响。方法回顾性分析2022年6月至2023年6月于常州市妇幼保健院生殖医学中心进行常规体外受精治疗的45个促排卵周期,每个周期获取的前10枚卵母细胞用于短时受精,剩余的卵母细胞则进行长时受精。短时受精组的450个卵母细胞在受精4.5 h后进行脱颗粒,长时受精组的663个卵母细胞则在受精18 h后进行脱颗粒,随后分析比较不同组卵母细胞的受精、卵裂胚发育及囊胚发育情况。结果不同受精时长的卵母细胞的受精差异无统计学意义(P>0.05),第三天优质卵裂胚率及囊胚发育的结局差异无统计学意义(P>0.05)。结论在排除受精失败的前提下,长时受精与短时受精有着相似的卵母细胞受精情况及胚胎发育结局,长时受精仍然具有一定的应用价值。Objective To investigate the effect of short-time fertilization and long-time fertilization to the fertilization and development outcomes of homologous oocytes.Methods A retrospective analysis of 45 IVF cycles was conducted on in Reproductive Medicine Center of Changzhou Maternal and Child Health Care Hospital from June 2022 to June 2023.The first 10 oocytes obtained in each cycle were used for short-term fertilization,and the remaining oocytes were used for long-term fertilization,450 oocytes were fertilized for 4.5 h in short-time fertilization group,while the other 663 oocytes fertilized for 18 h were in long-time fertilization group.The fertilization and embryo development outcomes of the two groups were observed and analyzed.Results There was no difference on fertilization rate among the two groups(P>0.05),also there was no significant difference in good quality embryo rate on day 3 and blastocyst development outcome(P>0.05).Conclusion Long-time fertilization and short-time fertilization have the similar results on fertilization rate and embryo development outcomes of homologous oocytes,without regard to the fertilization failure,long-time fertilization is still worthy in assisted reproductive technology.

关 键 词：同源卵母细胞 短时受精 长时受精 胚胎发育 氧化应激 

分 类 号：R714.13[医药卫生—妇产科学]