PEG处理对山梨种子热休眠的预防作用研究  

The Preventive Effect of PEG Treatment on Thermal Dormancy of Pyrus ussuriensis Seeds

作　　者：董博文 朱美如 刘婷[1] 张鹏[1] Dong Bowen;Zhu Meiru;Liu Ting;Zhang Peng(College of Forestry,Northeast Forestry University,Harbin Heilongjiang 150040,China)

机构地区：[1]东北林业大学林学院,黑龙江哈尔滨150040

出　　处：《西南林业大学学报（自然科学）》2024年第6期21-28,共8页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家自然科学基金项目(31870615)资助。

摘　　要：为探究PEG引发和渗透调节处理对山梨种子发芽指标和内源激素含量的影响,以解除初生休眠的山梨种子为实验材料,采用不同质量分数(5%、25%、50%)PEG溶液引发处理不同时间(0.5、1、2 d)或渗透处理不同时间(1、3、7 d)后,测定不同引发或渗透处理后的山梨种子在高温(25℃)下的萌发表现,并选择效果显著的处理测定种子内源激素变化。结果表明:25%PEG引发和渗透处理1 d均能够很好地减弱山梨种子热休眠的诱导,达到预防的效果。PEG引发处理减弱种子热休眠的效果好于渗透处理采用质量分数25%的PEG溶液引发处理1 d时,种子发芽率较CK提高57%,发芽指数提高0.65,平均发芽时间缩短7.88 d。引发处理通过增加萌发早期种子内源乙烯和GA3含量、减少ABA含量来预防山梨种子进入热休眠。To investigate the effects of PEG priming and osmotic treatment on the induction of the germination indicators and endogenous hormone content of Pyrus ussuriensis seeds,the primary dormancy released seeds of P.ussuriensis was used as experimental materials,the germination performance of P.ussuriensis seeds treated with different mass fractions(5%,25%,50%)of polyethylene glycol(PEG)solution for different durations(0.5,1,2 d)or osmotic treatment for different durations(1,3,7 d)at high temperature(25℃)was studied then select treatments with significant effects to measure changes in endogenous hormones in seeds.The results showed that both 25%PEG priming and osmotic treatment for 1 day could effectively weaken the induction of thermal dormancy in P.ussuriensis,achieving a preventive effect.The effect of PEG priming treatment on reducing seed thermal dormancy is better than that of osmotic treatment.When treated with 25%PEG solution for 1 day,the germination rate of seeds was increased by 57%,the germination index was increased by 0.65,and the average germina-tion time was shortened by 7.88 d.PEG priming can prevent P.ussuriensis seeds from entering thermal dormancy by increasing the endogenous ETH and GA3 content in the early germination stage and reducing ABA content.

关 键 词：山梨 种子萌发 热休眠 渗透处理 内源激素 

分 类 号：S722.1[农业科学—林木遗传育种]