云南欧洲甜樱桃品种SSR鉴定及遗传多样性分析  

Varieties Identification and Genetic Diversity Analysis of Sweet Cherry in Yunnan Province by SSR Markers

作　　者：杨跃仙 段利武 卯梅华 王毅 马山俊 Yang Yuexian;Duan Liwu;Mao Meihua;Wang Yi;Ma Shanjun(Yunnan Forestry Technological College,Kunming Yunnan 650224,China;Key Laboratory of National Forestry Administration on Conservation of Rare,Endangered&Endemic Forest Plants/Yunnan Provincial Key Laboratory of Cultivation and Exploitationof Forest Plants/Yunnan Academy of Forestry and Grassland,Kunming Yunnan 650201,China)

机构地区：[1]云南林业职业技术学院,云南昆明650224 [2]云南省林业和草原科学院,云南省森林植物培育与开发利用重点实验室,国家林业局云南珍稀濒特森林植物保护和繁育重点实验室,云南昆明650201

出　　处：《西南林业大学学报（自然科学）》2024年第6期72-80,共9页Journal of Southwest Forestry University：Natural Sciences

基　　金：云南省教育厅科学研究基金项目(2023J1620)资助;云南省“兴滇英才支持计划”教学名师项目(YNWR−JXMS−2019−038)资助。

摘　　要：为了解云南省目前引种和栽培的欧洲甜樱桃品种情况及其遗传多样性状况,经过SSR标记的筛选,对云南省昆明市、马龙县、石林县、牟定县不同基地和资源圃的85份欧洲甜樱桃品种进行基因型鉴定和遗传多样性分析。结果表明:共筛选出16个稳定性好、高度多态性的SSR标记;85份欧洲甜樱桃品种中有71份品种都有不同程度的重复,剔除同物异名和异物同名的品种后,筛选出31份欧洲甜樱桃品种;31份品种用16个SSR标记共扩增出74个等位片段,有效等位基因(Ne)的大小为1.1027~3.8363,平均值为2.70,香农指数(I)范围为0.2245~1.5151,平均值1.1295。观测杂合度(Ho)的范围为0.0968~0.7742,平均值0.6250,多态信息含量(PIC)的范围为0.0931~0.7393,平均值0.5953。该研究结果表明云南省85份甜樱桃品种实为31个基因型,遗传多样性水平中等偏高。The purpose of this study was to identify variety and analyze the genetic diversity of sweet cherry varieties introduced in Yunnan Province.Based on the screening of SSR markers reported in the literature,genotype identification and genetic diversity analysis were conducted on 85 sweet cherry varieties from different bases and resource nurseries in Kunming City,Malong County,Shilin County and Mouding County of Yunnan Province.The findings indicated that 16 SSR markers with good stability and polymorphism were selected;a total of 71 sweet cherry varieties in the 85 ones were duplicated to different degrees,and a sweet cherry variety list containing 31 varieties was sorted out after eliminating the inconsistent names;a total of 74 alleles were amplified with the 16 SSR markers,the size of effective allele(Ne)ranged from 1.1027–3.8363 with a mean value of 2.70,and the Shannon index(I)ranged from 0.2245–1.5151 with a mean value of 1.1295.The observed heterozygosity(Ho)ranged from 0.0968 to 0.7742 with a mean value of 0.6250,and the polymorphic information content(PIC)ranged from 0.0931 to 0.7393 with a mean value of 0.5953.The 85 sweet cherry varieties in Yunnan Province actually have 31 genotypes,with a medium to high level of genetic diversity.

关 键 词：甜樱桃 品种鉴定 遗传多样性 SSR 

分 类 号：S662.5[农业科学—果树学]