诱导山苍子胚性愈伤组织的影响因素研究  

The Factors Influencing the Embryogenic Callus Induction of Litsea cubeba

作　　者：毛卓佳 易敏 蒋润迪 陈尚钘 梁擎宇 吴祖明 王宗德 范国荣 付丹 汪加魏 Mao Zhuojia;Yi Min;Jiang Rundi;Chen Shangxing;Liang Qingyu;Wu Zuming;Wang Zongde;Fan Guorong;Fu Dan;Wang Jiawei(Jiangxi Provincial Key Laboratory of Subtropical Forest Resources Cultivation,College of Forestry,Jiangxi Agricultural University,Nanchang Jiangxi 330045,China;East China Woody Fragrance and Flavor Engineering Research Center of NFGA,Nanchang Jiangxi 330045,China;Yunnan Vocational and Technical College of Agriculture,Kunming Yunnan 650031,China)

机构地区：[1]江西农业大学林学院亚热带森林资源培育江西省重点实验室,江西南昌330045 [2]国家林业草原木本香料(华东)工程技术研究中心,江西南昌330045 [3]云南农业职业技术学院,云南昆明650031

出　　处：《西南林业大学学报（自然科学）》2024年第6期184-193,共10页Journal of Southwest Forestry University：Natural Sciences

基　　金：江西省林业局樟树研究专项(2020CXZX07−2020−01−03)资助;国家重点研发计划项目(2017YFD0601303)资助;江西省重大科技研发专项(20203ABC28W016)资助;江西省级大学生创新创业训练计划项目(S202210410045)资助。

摘　　要：以山苍子优良单株的近成熟种胚为外植体,分析不同消毒方式、激素组合、碳源、种源对胚性愈伤组织诱导的影响,筛选出山苍子胚性愈伤组织形成的最佳诱导条件。结果表明:以75%乙醇消毒内果皮5 min,无菌水冲洗5次,污染率最低为0.00%;江西省南昌市为胚性愈伤组织诱导的最佳种源,诱导率达7.04%;添加外源激素1.0 mg/L 6−BA+0.2 mg/L 2,4−D+0.5 mg/L IAA的胚性愈伤组织诱导率最高,达17.50%。成功诱导了山苍子胚性愈伤组织,并在培养基MS+0.5 mg/L 6−BA+0.1 mg/L 2,4−D+0.25 mg/L IAA中实现胚性愈伤组织的增殖与体细胞胚的分化,进而实现了体胚的萌发。In order to screen out the best induction conditions for embryogenic calls formation of Litsea cubeba,nearly mature seed embryos of L.cubeba good single plants were used as explants to analyze the effects of different disinfection methods,hormone combinations,carbon source type and seed source type on the embryogenic calls induction.The results showed that sterilizing the endocarp with 75%ethanol for 5 min and rinsing with sterile water for 5 times resulted in the lowest contamination rate of 0.00%;Nanchang City,Jiangxi Province,was the best seed source location for embryogenic calls induction,with an induction rate of 7.04%;and the addition of the exogenous hormone 1.0 mg/L 6−BA+0.2 mg/L 2,4−D+0.5 mg/L IAA had the highest rate of embryogenic calls,with a total induction rate of 17.50%.In this study,we successfully induced embryogenic calls of L.cubeba and achieved proliferation of embryogenic calls and differentiation of somatic embryos in medium MS+0.5 mg/L 6−BA+0.1 mg/L 2,4−D+0.25 mg/L IAA,and then realized the germination of somatic embryos.

关 键 词：山苍子 体细胞胚胎 愈伤组织 激素 增殖 

分 类 号：S722.3[农业科学—林木遗传育种]