基于LC−MS的樟叶越桔愈伤组织次生代谢成分分析  

LC−MS Analysis of Secondary Metabolites in the Callus of Vaccinium dunalianum

作　　者：王玮[1] 李楚然 武波晓 刘云[2] 杨晓琴[1] 朱国磊 赵平[1,2] Wang Wei;Li Churan;Wu Boxiao;Liu Yun;Yang Xiaoqin;Zhu Guolei;Zhao Ping(Key Laboratory of National Forestry and Grassland Administration on Highly-efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming Yunnan 650233,China;Key Laboratory of Ministry of Education for Forest Resources Conservation and Utilization in the Southwest Mountains of China,Kunming Yunnan 650233,China)

机构地区：[1]西南林业大学西南地区林业生物质资源高效利用国家林业和草原局重点实验室,云南昆明650233 [2]西南林业大学西南山地森林资源保育与利用教育部重点实验室,云南昆明650233

出　　处：《西南林业大学学报（自然科学）》2024年第6期194-199,共6页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家自然科学基金项目(32060107,32060327)资助。

摘　　要：以樟叶越桔健康愈伤组织为研究对象,采用LC−MS联用技术,对樟叶越桔愈伤组织的甲醇提取物进行分析。结果表明:正离子模式下共检测出14类共281个化合物,以萜类、生物碱类、有机酸、杂环化合物、糖苷类和醇类为主,化合物数量分别为58、41、28、24、22个和13个,相对含量分别为16.866%、13.711%、6.108%、10.138%、12.488%和17.513%;负离子模式下检测出13类共71个化合物,以生物碱类、有机酸、糖苷类、萜类和酯类为主,化合物数量分别为14、9、9、7个和7个,相对含量分别为5.839%、72.653%、3.423%、2.062%和2.735%,其中丙酮酸的相对含量高达47.474%。研究结果可为进一步开展樟叶越桔愈伤组织中主要化学成分的生产调控等研究提供参考。The investigation focused on the healthy callus of Vaccinium dunalianum,and the methanol content was analyzed using LC−MS technology.The results showed that a total of 281 compounds divided into 14 categories were detected in the positive ion mode,which mainly including 58 terpenoids,41 alkaloids,28 organic acids,24 heterocyclic compounds,22 glycosides and 13 alcohols,with a relative content of 16.866%,13.711%,6.108%,10.138%,12.488%and 17.513%,respectively.A total of 71 compounds divided into 13 categories were detected in the negative ion mode,among which 14 alkaloids,9 organic acids,9 glycosides,7 terpenoids and 7 esters were the main components with a relative content of 5.839%,72.653%,3.423%,2.062%and 2.735%,respectively.Especially,the relative content of pyruvic acid was the highest at 47.474%.The results will provide a basis for the further regulation of the major chemical constituents in the callus of V.dunalianum.

关 键 词：樟叶越桔 愈伤组织 化学成分 次生代谢 LC−MS 

分 类 号：TQ041[化学工程]