贵州省多重耐药沙门菌的分子流行病学特征及β-内酰胺类耐药基因分析  

Molecular epidemiological characteristics and analysis of β-lactam resistance genes of multi-drug resistant Salmonella,Guizhou

作　　者：文永仙 龙利 游旅[3] 刘艳敏 王铭 汪俊华[1] 韦小瑜 WEN Yong-xian;LONG Li;YOU Lv;LIU Yan-min;WANG Ming;WANG Jun-hua;WEI Xiao-yu(School of Public Health,the Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,Guizhou Medical University,Guiyang,Guizhou 561113,China;不详)

机构地区：[1]贵州医科大学公共卫生与健康学院,环境污染与疾病监控教育部重点实验室,贵州贵阳561113 [2]铜仁市疾病预防控制中心 [3]贵州省疾病预防控制中心

出　　处：《现代预防医学》2024年第22期4051-4056,4090,共7页Modern Preventive Medicine

基　　金：贵州省科技支撑计划项目(黔科合支撑[2020]4Y143号);贵州省科技基金计划项目(黔科合基础[2017]1094);贵州省传染病预防与控制人才基地科研团队项目(RCJD2104);贵州省卫生健康委科学基金项目(gzwkj2023-498)。

摘　　要：目的了解贵州省多重耐药沙门菌临床分离株的分子流行病学特征,明确菌株携带β-内酰胺酶耐药基因的情况,为贵州省多重耐药沙门菌的防治提供参考依据。方法以70株多重耐药沙门菌为研究对象,采用微量肉汤稀释法对其进行抗生素敏感性分析,采用多位点序列分型(Multilocus sequence typing,MLST)方法进行分子流行病学分析,应用PCR方法检测多重耐药沙门菌β-内酰胺酶耐药基因的携带情况。结果贵州省70株多重耐药沙门菌以同时对4~5类抗生素耐药为主,占64.3%,有4.3%的菌株出现了泛耐药。70株多重耐药沙门菌被分为15个序列型,分属于13个eBurst Group,其中ST34、ST11和ST19是最常见ST型。ST型别最多的是铜仁市,部分地区出现独有的ST型,不同优势ST型其耐药谱不同。80.0%菌株至少携带一种β-内酰胺类耐药基因,其中bla_(TEM)(78.6%)最常见,bla_(CTX-M)基因占2.9%,包括bla_(CTX-M-55)和bla_(CTX-M-64)亚型,bla_(SHV)、bla_(OXA-2)和bla_(PER)基因未检出。结论贵州省多重耐药沙门菌ST34、ST11和ST19为常见序列型,不同地区多重耐药沙门菌有一定的同源性,这些菌株可能在不同地区进行了传播,对β-内酰胺类抗生素耐药主要由blaTEM介导。Objective To understand the molecular epidemiological characteristics of multi-drug-resistant(MDR)Salmonella clinical isolates in Guizhou Province and clarify the carrying of β-lactamase resistance genes to provide a reference basis for the prevention and treatment of MDR Salmonella in Guizhou province.Methods A total of 70 MDR Salmonella were used for the study,antibiotic susceptibility analyses of Salmonella were carried out using the micro broth dilution method,molecular epidemiological analyses were carried out using the method of multilocus sequence typing(MLST).Polymerase Chain Reaction was used to detect the β-lactamase resistance genes in the MDR Salmonella strains.Results The 70 MDR Salmonella strains in Guizhou province were predominantly resistant to antibiotics of 4-5 classes at the same time,accounting for 64.3%of the total,even 4.3%of the strains showed extensive drug-resistance.The 70 MDR Salmonella strains contained 15 sequence types,belonging to 13 eBurst groups,with ST34,ST11,and ST19 being the most common ST types.The most ST type in Tongren City,and unique ST type appeared in some cities,with different dominant ST type with different resistance profiles.The detection of β-lactamase gene showed that 80.0%of the strains carried at least one of β-lactam-resistant genes,with the most common one being bla_(TEM)(78.6%).The bla_(CTX-M) gene was detected in 2.9%strains,and the resistant genes subtypes were bla_(CTX-M-55) and bla_(CTX-M-64).The bla_(SHV),bla_(OXA-2),and bla_(PER) genes were not detected.Conclusion The MDR Salmonella ST34,ST11 and ST19 as common sequence types in Guizhou province,and there was a certain degree of homology among MDR Salmonella in different regions,and that these strains may have been transmitted in different regions.Theβ-lactam antibiotics was mainly mediated by blaTEM.

关 键 词：沙门菌 多重耐药 多位点序列分型 Β-内酰胺酶基因 

分 类 号：R378.22[医药卫生—病原生物学]