Study on chemical constituents and biological activities of Sanghuangporus vaninii under static magnetic field  

静磁场作用下瓦尼桑黄的化学成分及其生物活性研究

作　　者：LIU Xuwen ZHOU Jie YE Dongyan LI Qianhui YIN Shuren ZHANG Suyi BAI Helong 刘旭雯;周洁;叶董艳;李芊慧;尹树仁;张夙一;白鹤龙(长春师范大学化学学院,吉林长春130032)

机构地区：[1]College of Chemistry,Changchun Normal University,Changchun 130032,China

出　　处：《分子科学学报》2024年第4期353-357,共5页Journal of Molecular Science

基　　金：supported by the Natural Science Foundation of Jilin Province(20220402050 GH,JJKH20220826KJ);Natural Science Foundation of Changchun Normal University(2020004).

摘　　要：The chemical composition and biological activity of Sanghuangporus vaninii solid culture treated by static magnetic field were studied.The compounds were isolated and purified using ultrasonic extraction and semi-preparative liquid chromatography.Their structures were identified through spectral data,and the inhibitory activities againstα-amylase,α-glucosidase and pancreatic lipase were evaluated.Three compounds,5,7-dihydroxy-3,4'-dimethoxyflavone(1),D-(+)-Trehalose(2),pinolenic acid(3),were extracted from the petroleum ether layer.Comparison of peak heights indicated a significantly higher content of compounds subjected to a 4 mT static magnetic field compared to those untreated.Activity tests revealed that compounds 1 and 2 exhibited strong anti-α-amylase and anti-α-glucosidase activities.Specifically,compound 1 had inhibition rates of(65.37±0.05)%and(73.81±0.12)%after 4 mT treatment,compared to(57.26±0.11)%and(65.33±0.14)%without.Compound 2 showed inhibition rates of(68.61±0.12)%and(65.38±0.09)%with the magnetic field,versus(60.71±0.06)%and(56.18±0.02)%without.Compound 3 displayed a notable inhibitory effect on pancreatic lipase,with rates of(60.83±0.03)%after 4 mT treatment and(53.77±0.09)%without.This study demonstrates that the static magnetic field enhances the chemical content and bioactivity of Sanghuangporus vaninii solid culture,providing a basis for its further development and application.研究了经静磁场处理的瓦尼桑黄固体培养物的化学成分和生物活性。采用超声提取以及半制备液相色谱法分离纯化,通过波谱数据鉴定化合物的结构,并评估了它们对α-淀粉酶、α-葡萄糖苷酶和胰脂肪酶的抑制活性。从瓦尼桑黄提取物的石油醚层中分离得到3个化合物,分别是5,7-dihydroxy-3,4'-dimethoxyflavone(1),D-(+)-Trehalose(2),pinolenic acid(3)。峰高比较表明,与未经处理的样品相比,经4 mT静磁场处理后的样品中化合物含量明显较高。活性测试表明,化合物1和2具有很强的抗α-淀粉酶和抗α-葡萄糖苷酶活性。具体而言,化合物1经4 mT静磁场处理后对2种酶的抑制率分别为(65.37±0.05)%和(73.81±0.12)%,而未经静磁场处理时的抑制率分别为(57.26±0.11)%和(65.33±0.14)%。化合物2在静磁场作用下对2种酶的抑制率分别为(68.61±0.12)%和(65.38±0.09)%,而无静磁场作用时的抑制率分别为(60.71±0.06)%和(56.18±0.02)%。化合物3对胰脂肪酶有显著的抑制作用,经4 mT静磁场处理后的化合物3对胰脂肪酶的抑制率为(60.83±0.03)%,而无静磁场时的抑制率为(53.77±0.09)%。本研究表明,静磁场能提高瓦尼桑黄固体培养物的化学成分和生物活性,为其进一步开发和应用提供了基础。

关 键 词：Sanghuangporus vaninii chemical component static magnetic field Α-AMYLASE Α-GLUCOSIDASE pancreatic lipase 

分 类 号：R284.1[医药卫生—中药学] TQ28[医药卫生—中医学]