模拟微重力环境维生素B1对小鼠脾淋巴细胞功能的影响  

Effects of vitamin B1 on function of splenic lymphocytes of mice in simulated microgravity

作　　者：司少艳 吴莹莹 秦亚亚 尚颖 马小淯 宋淑军 SI Shaoyan;WU Yingying;QIN Yaya;SHANG Ying;MA Xiaoyu;SONG Shujun(Department of Comprehensive Basic Experiment,Strategic Support Force Medical Center,Beijing 100101,China)

机构地区：[1]战略支援部队特色医学中心综合基础研究室,北京100101

出　　处：《中国免疫学杂志》2024年第12期2496-2499,2505,共5页Chinese Journal of Immunology

基　　金：战略支援部队特色医学中心科研课题(19ZX13)。

摘　　要：目的:了解维生素B1对模拟微重力环境淋巴细胞功能的影响。方法:分离小鼠脾细胞,采用旋转细胞培养系统模拟微重力效应,用有丝分裂源刀豆球蛋白A刺激淋巴细胞,并用不同浓度的维生素B1处理细胞,检测淋巴细胞的增殖指数及培养上清细胞因子水平。结果:模拟微重力效应可使脾淋巴细胞的增殖能力受到抑制,细胞因子水平下降,而维生素B1呈剂量依赖性地促进模拟微重力培养的淋巴细胞增殖及细胞因子分泌。结论:维生素B1可能通过调节淋巴细胞的增殖功能及细胞因子的分泌减轻模拟微重力环境对淋巴细胞的抑制作用。Objective:To understand the effect of vitamin B1 on lymphocyte function in simulated microgravity.Methods:Splenocytes of mice were isolated,and the rotatary cell culture system was used to simulate microgravity.Lymphocytes were stimulated with mitotic agents Concanavalin A,and cells were treated with different concentrations of vitamin B1,proliferation indexes of lymphocytes and levels of cytokines in supernatant were detected.Results:Simulated microgravity could inhibit proliferation of splenic lymphocytes,and decrease levels of cytokines,while vitamin B1 could promote lymphocyte proliferation and cytokines production in cells cultured in simulated microgravity in a dose dependent manner.Conclusion:Vitamin B1 may attenuate the inhibitory effect of simulated microgravity on lymphocytes by regulating cell proliferation and secretion of cytokines.

关 键 词：维生素B1 模拟微重力 淋巴细胞 细胞增殖 细胞因子 

分 类 号：R392.12[医药卫生—免疫学]