免疫性血小板减少症患者外周血CD4^(+)CD28-T细胞数量及功能分析  

Analysis of frequency and functions of CD4^(+)CD28-T cells in patients with immune thrombocytopenia

作　　者：李伟平 白自然[1] 田渝琴 尹春来 李霞 LI Weiping;BAI Ziran;TIAN Yuqin;YIN Chunlai;LI Xia(Department of Immunology,College of Basic Medical Science,Dalian Medical University,Dalian 116044,China;The Second Affiliated Hospital of Dalian Medical University,Dalian 116027,China)

机构地区：[1]大连医科大学基础医学院免疫教研室,大连116044 [2]大连医科大学附属第二医院,大连116027

出　　处：《中国免疫学杂志》2024年第12期2623-2627,共5页Chinese Journal of Immunology

基　　金：辽宁省自然科学基金面上项目(2021-MS-285)。

摘　　要：目的:研究免疫性血小板减少症(ITP)患者外周血CD4^(+)CD28-T细胞比例及功能,深入了解ITP的发病机制。方法:选取2021年1月至2022年6月于大连医科大学附属第二医院就诊的ITP患者30例及30例性别、年龄匹配的健康人作为对照,收集外周抗凝血提取单个核细胞(PBMC),流式细胞术分析PBMC中CD4^(+)CD28-T细胞比例及功能,ELISA检测ITP患者血浆中IL-18、IL-18BP含量,IL-18刺激PBMC,流式细胞术检测IL-18刺激前后CD4^(+)CD28-T细胞比例。结果:ITP患者外周血CD4^(+)CD28-T细胞比例明显高于健康对照组,且IFN-γ和TNF-α分泌能力更强;ITP患者血浆中IL-18含量上调;IL-18刺激可提高ITP患者PBMC中CD4^(+)CD28-T细胞比例。结论:ITP患者外周血中CD4^(+)CD28-T细胞比例升高,分泌炎症因子的能力更强。Objective:To investigate frequency and function of peripheral CD4^(+)CD28−T cells in immune thrombocytopenia(ITP)patients,to deeply understand pathogenesis of ITP.Methods:A total of 30 ITP patients treated in The Second Affiliated Hospital of Dalian Medical University from January 2021 to June 2022 were recruited,and 30 gender/age-matched healthy volunteers were served as control group.Peripheral anticoagulation was collected and mononuclear cells(PBMC)were extracted,frequencies and functions of CD4^(+)CD28−T cells in PBMC was analyzed by flow cytometry,plasma levels of IL-18 and IL-18BP in ITP patients were detected by ELISA,and frequencies of CD4^(+)CD28−T cells before and after treated with IL-18 were determined by flow cytometry.Results:Frequencies of CD4^(+)CD28−T cells in peripheral blood of ITP patients were significantly higher than that of healthy control group,and CD4^(+)CD28−T cells had a stronger ability to secrete IFN-γand TNF-α;level of IL-18 was higher in plasma from ITP patients;IL-18 could increase frequency of CD4^(+)CD28−T cells in PBMC of ITP patients.Conclusion:Frequency of CD4^(+)CD28−T cells in peripheral blood of ITP patients is significantly increased,and has stronger ability to secrete inflammatory factors.

关 键 词：ITP CD4^(+)CD28-T IL-18 

分 类 号：R588.2[医药卫生—内分泌]