基于UPLC-QTOF/MS的清开灵注射液过敏反应豚鼠血清代谢组学研究  

Ultra Performance Liquid Chromatography and QTOF/Mass Spectrometry-Based Research on Serum Metabonomics of Guinea Pigs with Anaphylaxis Induced by Qingkailing Injection(清开灵注射液)

作　　者：刘晓娟 陈娜[1] 刘莎莎 谢谦 陈凌[1] LIU Xiaojuan;CHEN Na;LIU Shasha;XIE Qian;CHEN Ling(Changsha Hospital for Maternal&Child Care Affiliated to Hunan Normal University,Changsha Hunan 410007,China;Hunan Provincial Maternal and Child Care Hospital,Changsha Hunan 410008,China)

机构地区：[1]湖南师范大学附属长沙市妇幼保健院,湖南长沙410007 [2]湖南省妇幼保健院,湖南长沙410008

出　　处：《中医药导报》2024年第11期22-25,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：长沙市妇幼保健院院级科研项目(202324-2)。

摘　　要：目的:利用代谢组学方法研究清开灵注射液过敏反应豚鼠血清样本,探索其可能存在的特征性生物标志物。方法:利用超高效液相色谱-四级杆飞行时间串联质谱(UPLC-QTOF/MS)技术对清开灵注射液组豚鼠和空白对照组豚鼠血清代谢指纹图谱展开分析,根据主成分分析(PCA)和正交信号校正偏最小二乘判别分析(OSC-PLS-DA)结果筛选出差异性代谢物。结果:清开灵注射液组与空白对照组分别呈现聚类分布,确认出5个生物标志物,包括硬脂酰溶血磷脂酰胆碱、肉豆蔻酰溶血磷脂酰胆碱、二氢神经鞘氨醇、蟾二烯羟酸内酯和3α,12β-二羟基-5β-胆烷酸。结论:建立了利用液相色谱-质谱技术进行清开灵注射液过敏反应豚鼠血清代谢组学研究的方法,为进一步认识清开灵注射液的过敏反应及中药注射剂安全性评价研究提供了新的思路。Objective:The metabonomics method was used to study the serum samples from guinea pigs sensitized by Qingkailing injection(QKL),in order to explore possible existent characteristic biomarkers.Methods:The serum metabolic fingerprinting of guinea pigs from control group and QKL group were analyzed by ultra-performance liquid chromatography coupled with quadrupole time-of-flight/mass spectrometry(UPLC-QTof/MS).The different metabolites were screened according to the result of the principal component analysis(PCA)and orthogonal signal correction-partial least square discriminate analysis(OSC-PLS-DA).Results:The cluster distribution was displayed in both QKL group and control group,and 5 compounds were initially identified as biomarkers,including LysoPC(18:0),LysoPC(14:0),Sphinganine,Bufadienolide,and Lagodeoxycholic.Conclusion:The method of serum metabolomics study of guinea pigs with anaphylaxis induced by Qingkailing injection has been constructed by using the technology of UPLC-QTof/MS which provide new ideas for the further understanding the allergic reaction of Qingkailing injection and the safety evaluation of traditional Chinese medicine injection(TCMI).It is conducive to promoting the clinical application of TCMI and the development of related industries.

关 键 词：清开灵注射液 过敏反应 超高效液相色谱-四级杆飞行时间串联质谱 代谢组学 生物标志物 豚鼠 

分 类 号：R285.5[医药卫生—中药学]