艾DUS测试指南的研制  

Development of DUS testing guidelines of Artemisia argyi

作　　者：陈昌婕 苗玉焕 万修福 郭兰萍[2] 刘大会 CHEN Chang-jie;MIAO Yu-huan;WAN Xiu-fu;GUO Lan-ping;LIU Da-hui(College of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065,China;State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Hubei Shizhen Laboratory,Wuhan 430065,China)

机构地区：[1]湖北中医药大学药学院,湖北武汉430065 [2]中国中医科学院中药资源中心道地药材品质保障与资源持续利用全国重点实验室,北京100700 [3]湖北时珍实验室,湖北武汉430065

出　　处：《中国中药杂志》2024年第21期5835-5842,共8页China Journal of Chinese Materia Medica

基　　金：国家重点研发计划项目(2023YFC3503804);湖北省自然科学基金创新群体项目(2023AFA032);中央本级重大增减支项目(2060302)。

摘　　要：艾为菊科蒿属多年生草本植物,以叶片入药。艾生产中存在着严重的种质不清、品种混杂等问题,亟须进行新品种保护工作。植物新品种特异性、一致性、稳定性测试(DUS测试)是植物新品种保护的基础,研制DUS测试指南是开展DUS测试的技术依据。为了研制艾的DUS测试指南,该实验以100份艾种质资源为研究对象,在艾的6个生育期对其农艺性状和药材品质性状进行了观察和测定,并对性状进行了分级和描述,共确定了53个测试性状,其中包括19个必测性状;涉及4个植株性状、2个根状茎性状、5个茎秆性状、3个分枝性状、29个叶片性状、3个花部性状、5个药材品质性状和2个其他性状;有16个质量性状、22个数量性状和15个假质量性状。从53个性状中确定了“出苗期”“植株-株型”“分枝-一级分枝部位”“茎-颜色”“中部叶-叶裂数”“现蕾期”“植株-高度”等7个分组性状;通过查找标准性状,最终确定了16个标准品种。该指南的研制对艾新品种的审查和保护、保障育种者权利、促进艾产业的发展具有重要意义。Artemisia argyi is a perennial herbaceous herb of the Artemisia family,with leaves for medical use.However,the germplasm of A.argyi is seriously unclear and mixed during production,and it is urgent to protect new varieties of A.argyi.The distinctness,uniformity,and stability(DUS)testing of the new varieties of plants is the basis for the protection of new varieties of plants,and the development of the DUS testing guidelines is the technical basis for DUS testing.To develop the DUS testing guidelines of A.argyi,A.argyi of 100 germplasm was used as the research objects,and their agronomic and medicinal quality characters were observed and measured during six growth stages,and each character was graded and described.A total of 53 test characters were determined,including 19 characters that must be tested;there were four plant characters,two rhizome characters,five stem characters,three branching characters,29 leaf characters,three floral characters,five medicinal quality characters,and two other characters.It also involved 16 quality characters,22 quantitative characters,and 15 pseudo-quantitative characters.Seven grouping characters were determined from 53 characters,including"emergence period""plant-plant type""branching-primary branching site""stem-color""middle leaf-number of leaf splits""budding period",and"plant-height".By searching for standard characters,16 standard varieties were ultimately determined.The preparation of this guideline was of great significance for the review and protection of new A.argyi varieties,the protection of breeders′rights,and the promotion of the development of A.argyi industry.

关 键 词：艾 DUS 测试指南 植物新品种保护 

分 类 号：R282.71[医药卫生—中药学]