鸡酪氨酸酶(TYR)基因启动子区序列结构特征分析  

Analysis of Structure Characteristics of Promoter Region Sequence of TYR Gene in Chicken(Gallus gallus)

作　　者：宋兴超 陈敏 安清明 孟金柱 赵园园 吴震洋 SONG Xingchao;CHEN Min;AN Qingming;MENG Jinzhu;ZHAO Yuanyuan;WU Zhenyang(Guizhou Provincial Key Laboratory for Biodiversity Conservation and Utilization in the Fanjing Mountain Region,College of Agroforesry Engineering and Planning,Tongren University,Tongren 554300,China;Animal Husbandry Technology Extension Station,Qiandongnan Miao and Dong Autonomous Prefecture,Kaili 556000,China)

机构地区：[1]铜仁学院农林工程与规划学院贵州省梵净山地区生物多样性保护与利用重点实验室,贵州铜仁554300 [2]黔东南苗族侗族自治州畜牧技术推广站,贵州凯里556000

出　　处：《特产研究》2024年第6期53-59,64,共8页Special Wild Economic Animal and Plant Research

基　　金：贵州省科技厅科技计划项目(黔科合基础-ZK〔2022〕一般559);黔东南州科技计划项目(黔东南科合J字〔2021〕54号);贵州省教育厅重点领域项目(黔教合KY字〔2020〕052)。

摘　　要：为解析鸡酪氨酸酶(Tyrosinase,TYR)基因启动子区序列的结构特征及其转录调控机制,本研究基于NCBI数据库中鸡TYR基因5'侧翼区与外显子1共计2944bp核苷酸序列,利用不同在线软件对其核心启动子区、基本调控元件、转录因子结合位点与CpG岛等结构开展生物信息学预测分析,运用DNASTAR.Lasergene 17.3和MEGA 5.0软件对不同物种TYR基因启动子区序列进行相似性分析和系统进化树构建。结果表明,起始密码子上游1500~2 100 bp为鸡TYR基因潜在的候选核心启动子区域;5'侧翼区1604bp存在1个潜在的转录起始位点C;该基因启动子区存在1个CAAT-box、9个TATA-box和15个E-box,不存在CpG岛结构域。综合多种在线软件预测鸡TYR基因启动子区存在C/EBPalp、GATA-1、Oct-1、TBP、Sp1、NF-1、AP1、SRF和NF-κB等转录因子结合位点。鸡TYR基因启动子区序列与环颈雉、火鸡、鹌鹑、绿头野鸭、棕硬尾鸭、凤头潜鸭、猪、普通牛、水牛、杂交牛、山羊、绵羊、赤鹿、白尾鹿、马鹿和林麝相似性在41.2%~89.7%之间。系统进化树表明,鸡与鹌鹑、环颈雉和火鸡亲缘关系较近,与猪亲缘关系较远。本研究结果可为进一步探明鸡色素沉积关联TYR基因的转录调控机制提供理论依据。In order to analyze the structure characteristics of promoter region sequence of TYR gene in chicken,and the transcriptional regulation mechanismwere preliminarily explored.Based on the 2944 bp nucleotide sequence of 5'UTR and exon 1 of chicken TYR gene registered in NCBI database,the core promoter region,basic regulatory elements,transcription factor binding sites and CpGisland were analyzed by means of a variety of online softwares.The similarity of TYR gene promoter sequence between chicken and other species,at the same time,phylogenetic tree construction were carried out used DNASTAR.Lasergene 17.3 and MEGA 5.0.The results showed that the 1500~2100 bp upstream of initiaton codon of chicken TYR gene was a possible candidate core promoter region,and one potential transcription initiation site C was found at 1604 bp of 5'UTR.There were one CAAT-box,nine TATA-boxes,and there was lack of CpG island in the promoter region of TYR gene.The sequence also contains some transcription factor binding sites,such as C/EBPalp,GATA-1,Oct-1,TBP,Sp1,NF-1,AP1,SRF and NF-κB.The similarity of promoter region sequence for TYR gene between chicken and ring necked pheasant,turkey,quail,mallard,ruddy duck,crested duck,pig,common cow,water buffalo,hybrid cow,goat,sheep,red deer,white tailed deer,red deer and forest musk deer was 41.2%~89.7%.Phylogenetic tree revealed that chicken was closer with quail,ring necked pheasant,turkey and the farest related was pig.This research results could provide theoretical basis for further revealing the transcriptional regulation mechanism of TYR gene for pigmentation trait of chicken.

关 键 词：鸡 酪氨酸酶基因 启动子区 转录因子 调控元件 

分 类 号：S831[农业科学—畜牧学]