姜黄素通过PI3K/AKT信号抑制甲状腺乳头状癌细胞增殖和诱导细胞凋亡  

Curcumin Inhibits Proliferation and Induces Apoptosis in Papillary Thyroid Cancer Cells via PI3K/AKT Signalling

作　　者：刘占鑫 王金西 LIU Zhanxin;WANG Jinxi(Department of Pathology,Handan Hangang Hospital,Handan 056001,China;Department of Thyroid Surgery,Handan First Hospital,Handan 056000,China)

机构地区：[1]邯郸邯钢医院病理科,河北邯郸056001 [2]邯郸市第一医院甲状腺外科,河北邯郸056000

出　　处：《特产研究》2024年第6期76-80,共5页Special Wild Economic Animal and Plant Research

摘　　要：本文旨在研究姜黄素对人甲状腺乳头状癌细胞(B-CPAP)PI3K/AKT信号通路的影响。采用CCK-8、平板克隆形成试验检测不同浓度姜黄素对B-CPAP细胞12、24、48 h的抑制作用;TUNEL检测不同浓度作用24 h后B-CPAP细胞凋亡的发生率;Western blot检测不同浓度姜黄素作用B-CPAP细胞后PI3K、p-AKT、AKT蛋白的表达;RT-PCR检测不同浓度姜黄素作用B-CPAP细胞后caspase-3、BAX、BCL-2 mRNA的表达。CCK-8试验证明,姜黄素对B-CPAP细胞增殖具有抑制作用,且随剂量增加而增强;TUNEL法表明,B-CPAP细胞凋亡随姜黄素剂量增加而显著增加。作用B-CPAP细胞后,PI3K、p-AKT、BCL-2表达水平降低,caspase-3、BAX表达水平上升。姜黄素可能通过下调PI3K/AKT信号通路抑制甲状腺乳头状癌细胞增殖和诱导细胞凋亡。To study the effects of curcumin on PI3K/AKT signaling pathway in human thyroid papillary cancer cells(B-CPAP).The inhibitory effects of curcumin on B-CPAP cells at 12,24 and 48 h were detected by CCK-8 and clone formation assay.The apoptosis rate of BCPAP cells was detected by TUNEL after 24 h treatment with different concentrations.Western blot analysis was performed to detect the expression of PI3K,p-AKT and AKT protein in B-CPAP cells treated with curcumin at different concentrations.The mRNA expression of caspase-3,BAX and BCL-2 in B-CPAP cells treated with curcumin at different concentrations was detected by RT-PCR.The CCK-8 assay showed that curcumin inhibited the proliferation of B-CPAP cells and increased with increasing doses.TUNELmethod showed that B-CPAP cell apoptosis was significantly increased with curcumin doses increasing.After treatment of B-CPAP cells,the expression levels of PI3K,p-AKTand BCL-2 were decreased,while the expression levels of caspase-3 and BAXwere increased.Curcumin may inhibit the proliferation and induce apoptosis of thyroid papillary carcinoma cells by down-regulating PI3K/AKT signaling pathway.

关 键 词：姜黄素 人甲状腺乳头状癌细胞 PI3K信号通路 细胞凋亡 

分 类 号：R285[医药卫生—中药学]