李资源果实主要性状的SSR标记关联分析及指纹图谱构建  

SSR Marker Association Analysis and Fingerprint Construction of Main Traits of Plum Fruit

作　　者：岳林清 吴怡超 何星星 向明燕 王进[2] 武峥[2] 朱世国 丁梦琦 张建[1] 方小梅 易泽林[1] YUE Linqing;WU Yichao;HE Xingxing;XIANG Mingyan;WANG Jin;WU Zheng;ZHU Shiguo;DING Mengqi;ZHANG Jian;FANG Xiaomei;YI Zelin(School of Agriculture and Biotechnology,Southwest University,Chongqing 400715,China;Fruit Research Institute of Chongqing Academy of Agricultural Sciences,Chongqing 401329,China;Chongqing Seed Station,Chongqing 400060,China)

机构地区：[1]西南大学农学与生物科技学院,重庆400715 [2]重庆市农业科学院果树研究所,重庆401329 [3]重庆市种子站,重庆400060

出　　处：《园艺学报》2024年第11期2495-2509,共15页Acta Horticulturae Sinica

基　　金：重庆市自然科学基金项目(CSTB2022NCQ-msx1034);重庆市种子站基金项目(4322200266)。

摘　　要：2022和2023年对收集的102份李资源的11个果实性状进行鉴定,其中单果质量的变异系数最大,可食率和果形指数的变异系数较小。利用78对多态性引物在供试材料中共扩增出405个等位变异,多态性信息含量(PIC)为0.06~0.80,平均值0.53。对SSR标记和11个主要性状进行关联分析,共检测到55个SSR标记与主要性状极显著关联,表型变异解释率为4.01%~14.76%。其中有29个标记在两年的GLM和MLM模型下,出现2次及以上与同一性状极显著关联。筛选出可区分102份供试材料的27对核心引物,并构建了102个李种质的指纹图谱。A study on eleven fruit features generated from 102 plum resources was carried out in 2022 and 2023.The coefficient of variation for fruit weight was the highest,while edible ratio and fruit shape indices had lower coefficients.A total of 405 allelic variants were amplified in the test material using 78 pairs of polymorphic primers,with polymorphism information content(PIC)ranging from 0.06 to 0.80 and a mean value of 0.53.An association analysis revealed that 55 SSR markers had highly significant associations with 11 major traits,explaining 4.01%–14.76%of the phenotypic variance.Among the test materials,29 exhibited two or more highly significant associations with traits in two years under GLM and MLM models.From 78 primers,27 pairs of primers were selected to distinguish 102 materials,and the fingerprints of 102 plum resources were constructed.

关 键 词：李 SSR 关联分析 指纹图谱 

分 类 号：S662.3[农业科学—果树学]