新德里番茄曲叶病毒侵染性克隆构建及甜瓜品种抗病性鉴定  

Construction of Tomato Leaf Curl New Delhi Virus Infectious Clone and Its Application in Evaluation for Melon Disease Resistance

作　　者：刘莉铭[1] 耿艳飞 郝芳敏 康保珊[1] 吴会杰[1] 古勤生[1] LIU Liming;GENG Yanfei;HAO Fangmin;KANG Baoshan;WU Huijie;GU Qinsheng(Key Laboratory of Fruit Breeding Technology of Ministry of Agriculture and Rural Affairs,Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China;Ningbo Key Laboratory of Characteristic Horticultural Crops in Quality Adjustment and Resistance Breeding,Ningbo,Zhejiang 315040,China)

机构地区：[1]中国农业科学院郑州果树研究所,农业农村部果树育种技术重点实验室,郑州450009 [2]宁波市特色园艺作物品质调控与抗性育种重点实验室,浙江宁波315040

出　　处：《园艺学报》2024年第11期2555-2564,共10页Acta Horticulturae Sinica

基　　金：现代农业产业技术体系建设专项资助(CARS-25-2023-G20);中国农业科学院科技创新工程项目(CAAS-ASTIP-2022-ZFRI-09);宁波市特色园艺作物品质调控与抗性育种重点实验室开放课题项目(NBYYL2023011)。

摘　　要：以新德里番茄曲叶病毒(tomato leaf curl New Delhi virus,ToLCNDV)甜瓜分离物Haimen 6(HM6)为研究对象,采用串联重复片段法构建该分离物的侵染性克隆,分析其在不同作物中的侵染性,同时对25份甜瓜品种进行抗性评价。结果显示,用分别含有1.57个DNA-A串联重复(1.57A)和1.43个DNA-B串联重复(1.43B)的侵染性克隆共浸润接种及用同时含有1.57A和1.43B的侵染性克隆单独接种甜瓜,植株的侵染率均达100%。将分别含有1.57A、1.43B的侵染性克隆共浸润接种其他作物,发现可以侵染本氏烟草、番茄、南瓜、西葫芦、瓠瓜、黄瓜、丝瓜,不能侵染西瓜。通过抗性评价,25份供试甜瓜材料中有3份表现为抗病,3份表现为感病,19份表现为高感病。In this study,a tomato leaf curl New Delhi virus(ToLCNDV)melon isolate Haimen 6(HM6)was used as template to obtain its infectious clone by tandem repeat fragment method,and its infectivity was examined in different crops.Meanwhile,the resistance of 25 Cucumis melo cultivars were evaluated.The results showed that whatever infectious clones containing 1.57-mer DNA-A tandem repeat(1.57A)and 1.43-mer DNA-B tandem repeat(1.43B),respectively,were co-infiltrated or the clone containing both 1.57A and 1.43B was directly inoculated with melon,and the infection rate of the plants could reach 100%.Infectious clones containing 1.57A and 1.43B,respectively,were co-infiltrated into other crops,and it was found that it could infect Nicotiana benthamiana,Solanum lycopersicum,Cucurbita moschata,Cucurbita pepo,Lagenaria siceraria,Cucumis sativus,and Luffa aegyptiaca,but could not infect Citrullus lanatus.Through resistance evaluation,it was found that 3 of the 25 tested melon materials showed resistance,3 materials showed susceptibility and 19 materials showed high susceptibility.

关 键 词：甜瓜 新德里番茄曲叶病毒 侵染性克隆 侵染性 抗性 

分 类 号：S652[农业科学—果树学]