霍山石斛多糖对动脉粥样硬化小鼠脂质代谢的影响及菌群结构改变  

Effects of Dendrobium huoshanense polysaccharides on lipid metabolism andgut microbiota alterations in atherosclerotic mice

作　　者：高晓燕 郑少君 戴军 陈乃东[1,2,3,4] GAO Xiao-yan;ZHENG Shao-jun;DAI Jun;CHEN Nai-dong(College of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China;Anhui Province Key Lab of Quality Evaluation and Improvement of Traditional Chinese Medicine;College of Biotechnology and Pharmaceutical Engineering,West Anhui University;Quality Evaluation and Quality Improvement of Chinese Medicine Lu’an Laboratory,Lu’an Anhui 237012,China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]中药质量评价与品质提升安徽省重点实验室 [3]皖西学院生物与制药工程学院 [4]中药质量评价与品质提升六安市实验室,安徽六安237012

出　　处：《中国药理学通报》2024年第12期2333-2340,共8页Chinese Pharmacological Bulletin

基　　金：安徽省中药质量评价与品质提升科研创新团队项目(No2022AH010090);安徽省高校协同创新中心项目(No GXXT-2022-079);海南省重点研发项目(No ZDYF2024 SHFZ076);高层次人才科研启动基金项目(No 00701092394)。

摘　　要：目的 探索霍山石斛多糖(Dendrobium huoshanense C.Z.Tang et S.J.Cheng polysaccharides,DHP)对动脉粥样硬化模型小鼠的降脂作用并探讨其对肠道菌群的调节作用及变化特征。方法 8周龄ApoE^(-/-)小鼠12只,随机分为H-1组和H-2组。两组均给予高脂饮食喂养(HFD),8周后H-2组DHP(400 mg·kg^(-1)·d^(-1))灌胃6周,H-1组灌胃生理盐水,期间两组分别于0、4、8、14周采集血样;并采集H-2组粪便,分别编号B、E、F、D;在第14周处死所有小鼠后收集肝肾组织。试剂盒检测血液指标,宏基因组学分析粪便样本。结果DHP灌胃后明显降低小鼠血脂水平(P<0.05)且对肝肾无明显损伤。宏基因组学结果显示,与B组相比,EF组Deferribacterota、Faecalibaculum相对丰度增加,Bacteroidota、Prevotella、Lachnoclostridium相对丰度降低(P<0.05);D组相较于EF组Bacteroidota、Prevotella、Lachnoclostridium相对丰度增加,Deferribacterota、Bacillota、Oscillibacter、Faecalibaculum相对丰度减少(P<0.05)。另Actinomycetota、Faecalibaculum_rodentiumen等菌群随HFD喂养时间的增加而增加(P<0.05),Parabacteroides和Lachnoclostridium等随HFD喂养时间的增加而减少(P<0.05)。结论 随着动脉粥样硬化的发展,小鼠肠道菌群结构发生明显变化;DHP的干预有改善小鼠菌群结构及脂质代谢的趋势。Aim To explore the lipid-lowering effect of Dendrobium huoshanense C.Z.Tang et S.J.Cheng polysaccharides(DHP),on atherosclerotic mice and its regulatory effect on intestinal flora.Methods Twelve 8-week-old ApoE^(-/-)mice were randomly divided into two groups:H-1 and H-2.Both groups were fed a high-fat diet(HFD).After eight weeks,the H-2 group received DHP(400 mg·kg^(-1)·d^(-1))for six weeks,while the H-1 group received normal saline.Blood samples were collected at 0,4,8 and 14 weeks.Feces from the H-2 group were collected and labeled as B,E,F and D,and liver and kidney tissues were collected post-mortem at week 14.Blood indexes were detected using kits,and fecal flora was analyzed by metagenomics.Results DHP significantly decreased blood lipid levels in mice(P<0.05)without causing significant liver and kidney damage.Metagenomic analysis showed that,compared with group B,the relative abundance of Deferribacterota and Faecalibaculum increased in EF group,while the relative abundance of Bacteroidota,Prevotella and Lachnoclostridium decreased(P<0.05).Compared with EF group,the relative abundance of Bacteroidota,Prevotella and Lachnoclostridium increased in group D,while the relative abundance of Deferribacterota,Bacillota,Oscilli-bacter and Faecalibaculum decreased(P<0.05).Actinomycetota,Faecalibaculum rodentium and other bacteria increased with longer HFD feeding time(P<0.05).Conversely,Parabacteroides and Lachnoclostridium decreased with increased HFD feeding time(P<0.05).Conclusions The intestinal flora structure of mice changes significantly with the progression of atherosclerosis.DHP intervention shows a potential to improve microflora structure and lipid metabolism in mice.

关 键 词：霍山石斛 多糖 动脉粥样硬化 宏基因组学 降脂 肠道菌群 

分 类 号：R-332[医药卫生] R284.1R322.45R344R543.5