维生素C对藏鸡精子低温及冷冻保存的保护作用研究  

Study on the protective effects of vitamin C on the cold and frozen preservation of Tibetan chicken sperm

作　　者：平措 郭玉 吴锦波 李志雄 熊燕[2] Pingcuo;GUO Yu;WU Jinbo;LI Zhixiong;XIONG Yan(Shannan City Animal Husbandry and Veterinary General Station,Shannan 856000,China;College of Animal Science and Veterinary Medicine,Southwest Minzu University,Chengdu 610041,China;Institute of Animal Science and Technology,Aba Tibetan and Qiang Autonomous Prefecture,Hongyuan 624402,China)

机构地区：[1]山南市畜牧兽医总站,西藏山南856000 [2]西南民族大学畜牧兽医学院,成都610041 [3]阿坝藏族羌族自治州畜牧科学技术研究所,四川红原624402

出　　处：《黑龙江畜牧兽医》2024年第22期58-62,67,133,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：四川省科技计划项目(2020YFSY0048);四川省自然科学基金面上项目(23NSFSC1804)。

摘　　要：为了探究维生素C对藏鸡精液低温及冷冻保存的作用,试验采集健康的25周龄藏鸡精液,在其稀释液或冻存液中添加不同浓度的维生素C后进行低温(4℃)与冷冻保存,检测保存后精子的活率、总超氧化物歧化酶(total superoxide dismutase,T-SOD)活力、丙二醛(malondialdehyde,MDA)含量。结果表明:用添加0.01 mg/mL维生素C的稀释液在4℃保存24 h和48 h后,藏鸡精子的活率显著提高(P<0.05),精子T-SOD活力有升高的趋势,而MDA含量有下降的趋势;冻存液中添加0.01 mg/mL的维生素C,冷冻解冻后精子活率及线粒体活性显著提高(P<0.05),同时精子T-SOD活力显著提高(P<0.05),MDA含量显著降低(P<0.05)。说明低温或冷冻保存藏鸡精液时添加0.01 mg/mL的维生素C可有效提高保存精子的活率及抗氧化能力。In order to explore the role of vitamin C in cold and frozen preservation of semen in Tibetan chicken,in the experiment,the semen of healthy 25-week-old Tibetan chickens was collected;different concentrations of vitamin C were added to its dilution or freezing solution for cold and frozen preservation.Sperm viability,total superoxide dismutase(T-SOD)activity and malondialdehyde(MDA)content were measured after preservation.The results showed that the sperm motility of Tibetan chicken was significantly increased after storage at 4℃for 24 h and 48 h with the dilution of 0.01 mg/mL vitamin C(P<0.05);sperm T-SOD motility tended to increase;however,the MDA content had a decreasing trend.0.01 mg/mL of vitamin C was added to the refrigerant solution,and sperm motility and mitochondrial activity were significantly increased after freezing and thawing(P<0.05).At the same time,the T-SOD motility of sperm was significantly increased(P<0.05);the content of MDA was significantly decreased(P<0.05).The results indicated that the addition of 0.01 mg/mL vitamin C to the semen of Tibetan chicken at cold and frozen preservation could effectively improve the motility and antioxidant capacity of preserved sperm.

关 键 词：维生素C 藏鸡 精液 低温保存 冷冻保存 精子抗氧化 

分 类 号：S831.2[农业科学—畜牧学]