止痛生肌膏促进慢性难愈性溃疡大鼠创面修复的机制研究  

Study on the mechanism of Zhitongshengji Ointment in promoting wound repair in rats with chronic refractory ulcers

作　　者：李顺宁 胡柳平 张锦生 黎鉴仪 LI Shun-ning;HU Liu-ping;ZHANG Jin-sheng;LI Jian-yi(Department of Vasculitis,the Affiliated Traditional Chinese Medicine Hospital,Guangzhou Medical University,Guangzhou 510130,Guangdong,China;不详)

机构地区：[1]广州医科大学附属中医医院脉管炎科,广东广州510130 [2]香港科技大学生命科学学院,中国香港999077

出　　处：《广东医学》2024年第11期1397-1403,共7页Guangdong Medical Journal

基　　金：广东省中医药局中医药科研项目(20211297)。

摘　　要：目的探讨止痛生肌膏促进大鼠慢性难愈性溃疡创面修复的作用机制。方法72只大鼠构建慢性难愈性溃疡模型,随机分为对照组(溃疡创面外敷以凡士林纱布)、观察组(溃疡创面外敷以止痛生肌膏),每组36只,每天换药1次。分别在3 d、7 d、14 d,观察创面愈合情况,通过数码相机结合Image J软件计算各组用药创面面积及愈合率,观察HE染色各组溃疡创面组织病理学变化,Masson染色结合ImageJ软件检测各组溃疡创面胶原蛋白表达量,蛋白免疫印迹法(WB)测量溃疡创面组织中碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)、表皮细胞生长因子(epidermal growth factor,EGF)及血管内皮生长因子(vascular endothelial growth factor,VEGF)蛋白的表达水平含量。结果与对照组相比,观察组创面面积、愈合率干预3 d后差异无统计学意义(P>0.05);7、14 d后,观察组创面面积缩小明显、创面愈合率明显提高(P<0.05)。HE染色、Masson染色观察显示,与对照组比较,观察组炎性细胞减少,新生毛细血管、胶原纤维及成纤维细胞增多,新生肉芽组织明显增多,上皮组织形成。干预后第3天,观察组大鼠创面组织中EGF蛋白表达水平高于对照组(P<0.05);bFGF、VEGF蛋白表达水平升高,但与对照组相比,差异无统计学意义(P>0.05)。与3 d比较,7 d及14 d创面组织中EGF、bFGF、VEGF水平含量显著提高,差异有统计学意义(P<0.01、P<0.05)。结论止痛生肌膏可上调溃疡组织中EGF、bFGF、VEGF等生长因子的表达,促进创面成纤维组织细胞增殖和血管新生,肉芽生长,这可能是其促进创面修复的作用机制。Objective To investigate the mechanism of Zhitongshengji Ointment in promoting the healing of chronic refractory ulcers in rats.Methods A total of 72 rats were used to establish a model of chronic refractory ulcers.They were randomly divided into two groups:the vaseline control group(control group)and the Zhitongshengji Ointment treatment group(observation group),with 36 rats in each group.The wounds were treated once daily,and wound healing was observed on days 3,7,and 14.Wound area and healing rate were calculated using a digital camera combined with ImageJ software.Histopathological changes in the ulcer wound tissue were observed by HE staining,and collagen protein expression was detected by Masson staining combined with ImageJ software.Western blot(WB)analysis was used to measure the expression levels of basic fibroblast growth factor(bFGF),epidermal growth factor(EGF),and vascular endothelial growth factor(VEGF)proteins in the ulcer wound tissues.Results Compared with the vaseline control group,no significant difference was observed after 3 days of intervention.However,on day 7 and day 14,the wound area in the observation group was significantly reduced,and the wound healing rate was significantly increased(P<0.05).HE and Masson staining showed that,compared to the control group,the observation group had fewer inflammatory cells,more new capillaries,collagen fibers,and fibroblasts,and significantly more granulation tissue and epithelial tissue formation.On day 3,the expression level of EGF protein in the wound tissues of the observation group was higher than that in the control group(P<0.05).Although the expression levels of bFGF and VEGF proteins increased,the differences were not statistically significant compared with the control group(P>0.05).As compared with day 3,the levels of EGF,bFGF,and VEGF in the wound tissues on day 7 and day 14 were significantly increased,with statistically significant differences(P<0.01,P<0.05).Conclusion Zhitongshengji Ointment can upregulate the expression of growth factors

关 键 词：止痛生肌膏 慢性难愈性溃疡 创面修复 生长因子 大鼠 

分 类 号：R632.1[医药卫生—外科学] G264.3[医药卫生—临床医学]