黄芪注射液对甲状腺癌荷瘤小鼠肿瘤抑制作用及组织凋亡相关蛋白表达与NF-κB通路的影响  

Tumor suppressive effects of Huangqi injection in thyroid cancer-bearing mice and its impact on the expression of tissue apoptosis related proteins and NF-κB pathway

作　　者：黄兰 孟雨萌 孟尚文 刘亚军[4] HUANG Lan;MENG Yumeng;MENG Shangwen(Department of Pharmacy,Shaanxi Province Mianxian Hospital,Mianxian Shaanxi 724200,China;Department of Psychosomatic Medicine,Xijing Hospital,Xi an Shaanxi 710032,China;Department of Pathology,Shaanxi Province Mianxian Hospital,Mianxian Shaanxi 724200,China)

机构地区：[1]勉县医院药剂科,陕西勉县724200 [2]西京医院心身医学科,陕西西安710000 [3]勉县医院病理科,陕西勉县724200 [4]宁强县天津医院药剂科,陕西宁强724400

出　　处：《四川中医》2024年第11期72-75,共4页Journal of Sichuan of Traditional Chinese Medicine

摘　　要：目的:分析黄芪注射液对甲状腺癌荷瘤小鼠肿瘤抑制作用及组织凋亡相关蛋白表达与核因子-κB(NF-κB)通路的影响。方法:将50只小鼠随机分为模型对照组、黄芪注射液(低、中、高剂量)组及顺铂组,每组为10只;每组小鼠均建立甲状腺癌荷瘤小鼠模型并进行体内抗肿瘤实验。模型对照组每日给予20mL/kg生理盐水灌胃,黄芪注射液(低、中、高剂量)组分别腹腔注射黄芪注射液(4.0g、8.0g、12.0g/kg),连续给药20d;顺铂组在第1d及11d腹腔注射顺铂(2.5×10^(-3) g/kg),其余时间给予20mL/kg生理盐水灌胃。记录肿瘤体积,20d处死小鼠,剥取瘤组织称重并计算抑瘤率;采用免疫组化法对肿瘤组织B淋巴细胞瘤2相关X蛋白(Bax)、B淋巴细胞瘤2(Bcl-2)、半胱天冬氨酸蛋白水解酶3(Caspase-3)、NF-κBp65蛋白表达进行检测。结果:与模型对照组比较,黄芪注射液(低、中、高剂量)组瘤重及肿瘤体积均显著降低( P <0.05),呈一定剂量依赖性。黄芪注射液(低、中、高剂量)组的抑瘤率分别为9.62%、22.75%、44.87%。与模型对照组比较,其余各组小鼠肿瘤组织Bax、Caspase-3蛋白表达明显上调,Bcl-2蛋白表达明显下调( P <0.05);黄芪注射液(低、中、高剂量)组Bax、Caspase-3蛋白随浓度的升高而升高,Bcl-2蛋白随浓度的升高而降低,两两组间比较差异有统计学意义( P <0.05)。与模型对照组比较,其余各组小鼠肿瘤组织NF-κBp65蛋白表达明显下调( P <0.05);与顺铂组比较,黄芪注射液(低、中、高剂量)组肿瘤组织NF-κBp65蛋白表达上调( P <0.05);黄芪注射液(低、中、高剂量)组NF-κBp65蛋白随浓度升高而下调( P <0.05)。结论:黄芪注射液可抑制甲状腺癌的增殖,其机制可能是与调控组织凋亡相关蛋白表达及NF-κB信号通路有关。Objective: To analyze the tumor suppressive effects of Huangqi injection in thyroid cancer-bearing mice, and its impact on the expression of tissue apoptosis related proteins and nuclear factors-κB(NF-κB) pathway. Methods: 50mice were randomly divided into the model control group, the(low-dose, medium-dose and high-dose) Huangqi injection groups, and the cisplatin group, with 10mice in each group. For each group, thyroid cancer-bearing mouse models were established and in vivo anti-tumor experiments were conducted. Mice in the model control group were given gavage with 20ml/kg of normal saline every day. Mice in the(low-dose, medium-dose and high-dose) Huangqi injection groups were respectively given intraperitoneal injection of 4.0g/kg, 8.0g/kg and 12.0g/kg of Huangqi injection, for 20d. Mice in the cisplatin group were given intraperitoneal injection of cisplatin(2.5×10 ^(-3) g/kg)) on day 1and day 11, and were given gavage with 20ml/kg of normal saline at other time. Tumor volume was recorded. 20d later, the mice were sacrificed and tumor tissues were collected to calculate the tumor inhibition rate. The protein expression of B cell lymphoma-2-associated X protein(Bax), B cell lymphoma-2(Bcl-2), caspase-3and NF-κBp65was detected by immunohistochemical method. Results: Compared with the model control group, tumor weight and volume of the(low-dose, medium-dose and high-dose) Huangqi injection groups were significantly lower( P <0.05), showing certain dose-dependent effect. The tumor inhibition rates in the(low-dose, medium-dose and high-dose) Huangqi injection groups were 9.62%, 22.75% and 44.87%, respectively. Compared with the model control group, the protein expression of Bax and caspase-3in tumor tissues of other groups was significantly up-regulated, while the expression of Bcl-2protein was significantly down-regulated( P <0.05). The protein levels of Bax and caspase-3in the(low-dose, medium-dose and high-dose) Huangqi injection groups increased, while the level of Bcl-2protein decreased with the increa

关 键 词：甲状腺癌 黄芪注射液 肿瘤抑制 核因子-κB 

分 类 号：R285.5[医药卫生—中药学]