足月胎膜早破合并羊膜腔感染外周血HBD-2、PCT、miRNA表达及意义  

Expressions of HBD-2,PCT and miRNA in peripheral blood of full-term premature rupture of membranes puerpera complicated with amniotic cavity infection and their significance

作　　者：李萍 李苓妙 刘云霞 叶晶 张茜 甄银芝 武子先 窦静 LI Ping;LI Ling-miao;LIU Yun-xia;YE Jing;ZHANG Qian;ZHEN Yin-zhi;WU Zi-xian;DOU Jing(Affiliated Hospital of Non commissioned Officer School of Army Medical University,Shijiazhuang,Hebei 050000,China)

机构地区：[1]陆军军医大学士官学校附属医院妇产科,河北石家庄050000 [2]陆军军医大学士官学校附属医院预防保健科,河北石家庄050000 [3]陆军军医大学士官学校附属医院药剂医工科,河北石家庄050000 [4]陆军军医大学士官学校附属医院野战内科教研室,河北石家庄050000

出　　处：《中华医院感染学杂志》2024年第21期3331-3335,共5页Chinese Journal of Nosocomiology

基　　金：河北省医学科学研究基金资助项目(20221905)。

摘　　要：目的探讨足月胎膜早破(PROM)合并羊膜腔感染患者外周血人β防御素2(HBD-2)、降钙素原(PCT)、微小核糖核酸(miR)-182、miR-200表达及意义。方法选取2020年6月-2023年6月陆军军医大学士官学校附属医院收治的足月PROM产妇136例,根据羊膜腔感染情况分为感染组28例和非感染组108例。比较两组血清HBD-2、PCT、外周血miR-182、miR-200水平及妊娠结局;分析四指标联合检测对足月PROM合并羊膜腔感染诊断价值。结果感染组血清HBD-2、PCT、外周血miR-182、miR-200水平比非感染组高(P<0.05)。绘制受试者工作特征(ROC)曲线发现,四指标联合检测诊断足月PROM合并羊膜腔感染曲线下面积(AUC)比各项单独检测高(P<0.05),敏感度85.70%,特异度82.40%。感染组孕妇剖宫产、产褥感染和新生儿感染的占比较非感染组高(P<0.05)。结论HBD-2、PCT、miR-182、miR-200参与足月PROM合并羊膜腔感染发生,联合检测有助于诊断足月PROM合并羊膜腔感染。足月PROM合并羊膜腔感染可引起不良妊娠结局。OBJECTIVE To explore the expressions of humanβ-defensin 2(HBD-2),procalcitonin(PCT),microribonucleic acid(miR-182)and miR-200 in peripheral blood of the full-term premature rupture of membranes(PROM)puerpera with amniotic cavity infection and analyze the significance.METHODS A total of 136 full-term PROM puerpera who were treated in Affiliated Hospital of Non commissioned Officer School of Army Medical University from Jun.2020 to Jun.2023 were enrolled in the study and were divided into the infection group with 28 cases and the non-infection group according to the status of amniotic cavity infection.The levels of serum HBD-2,PCT,peripheral blood miR-182,miR-200 and pregnancy outcomes were observed and compared between the two groups.The value of the joint detection of the four indexes in diagnosis of amniotic cavity infection of the full-term PROM puerpera was analyzed.RESULTS The levels of serum HBD-2,PCT,peripheral blood miR-182 and miR-200 of the infection group were significantly higher than those of the non-infection group(P<0.05).Receiver operating characteristic(ROC)curve analysis showed that the area under the curve(AUC)of the joint detection of the four indexes was significantly higher than that of the single detection in the diagnosis of amniotic cavity infection in the full-term PROM puerpera(P<0.05),with the sensitivity 85.70%,the specificity 82.40%.The proportions of the puerpera with cesarean section,puerperal infection and neonatal infection were significantly higher in the infection group than in the non-infection group(P<0.05).CONCLUSIONS HBD-2,PCT,miR-182 and miR-200 participate in the occurrence of amniotic cavity infection in the full-term PROM puerpera,and the joint detection of the indexes may facilitate the diagnosis of amniotic cavity infection.The full-term PROM complicated with amniotic cavity infection may result in the adverse pregnancy outcomes.

关 键 词：胎膜早破 羊膜腔感染 人Β防御素2 降钙素原 微小核糖核酸-182 微小核糖核酸-200 受试者工作特征曲线 

分 类 号：R714.433[医药卫生—妇产科学]