莪术二酮溶解大鼠深静脉血栓的纤溶作用  

Fibrinolytic effect of curdione in dissolving deep vein thrombosis in rats

作　　者：史寒冰 张腾 杜珊 管毓萱 黄燕[3] 夏泉[1,3] SHI Hanbing;ZHANG Teng;DU Shan;GUAN Yuxuan;HUANG Yan;XIA Quan(School of Pharmacy,Anhui Medical University,Hefei 230032,China;Department of Integrated Traditional Chinese and Western Medicine,Anhui Medical University,Hefei 230022,China;Department of Pharmacy,the First Affiliated Hospital of Anhui Medical University,Hefei 230022,China)

机构地区：[1]安徽医科大学药学院,合肥230032 [2]安徽医科大学中西医结合学系,合肥230022 [3]安徽医科大学第一附属医院药剂科,合肥230022

出　　处：《药学前沿》2024年第11期424-430,共7页China Pharmacist

基　　金：国家自然科学基金面上项目(82174011)。

摘　　要：目的探讨莪术二酮是否通过溶栓作用来抑制大鼠深静脉血栓(DVT)的形成。方法将25只大鼠随机分为5组:正常对照组、假手术组、模型组、莪术二酮低(60mg/kg)和高(120 mg/kg)剂量组,每组5只。采用下腔静脉结扎模型,给药1周后腹主动脉取血于枸橼酸钠抗凝管内,检测各组大鼠纤维蛋白降解产物(FDP)、D-二聚体(D-D)、纤溶酶原激活物抑制因子(PAI)-1、纤溶酶原(PLG)等纤溶指标;检测下腔静脉血栓重量和重量长度比值;苏木素-伊红(HE)染色法检测大鼠下肢静脉血栓的形成;Western Blot实验检测下腔静脉组织核因子(NF)-κB蛋白表达。结果各组大鼠血浆FDP水平差异无统计学意义(P>0.05);与模型组比较,莪术二酮给药组大鼠血浆D-D水平含量升高,PAI-1、PLG水平含量下降,且高剂量莪术二酮给药组的纤溶作用优于低剂量给药组(P<0.05);与模型组相比,莪术二酮给药组血栓重量/重量长度比、血栓重量、NF-κB蛋白表达量降低(P<0.05)。结论莪术二酮可能通过影响纤溶调节因子,从而抑制大鼠DVT的形成。Objective To investigate whether curdione inhibits the formation of deep vein thrombosis(DVT)in rats through thrombolytic effects.Methods Twenty-five rats were randomly divided into five groups,the normal control group,the sham operation group,the model group,the low dose group of curdione(60 mg/kg)and the high dose group of curdione(120 mg/kg),with five rats in each group.Using the inferior vena cava ligation model,blood was taken from the pulmonary aorta in a sodium citrate anticoagulant tube one week after drug administration.The coagulation and fibrinolytic indexes Fibrin Degradation Products,(FDP),D-dimer(D-D),plasminogen activator inhibitor(PAI)-1 and plasminogen(PLG)were detected in each group;the thrombus weight/weight-length ratio was detected;and the formation of venous thrombus in the lower limbs of rats was detected by hematoxylin-eosin(HE)staining nuclear factor;NF-κB protein expression in inferior vena cava tissues was dectected by Western Blot assay.Results There was no significant difference in plasma FDP levels among groups of rats(P﹥0.05);compared with the model group,the plasma FDP and D-D levels of rats in each dosing group were increased,the plasma PAI-1 and PLG levels were elevated,and the fibrinolytic effect was better in the group with high dose of curdione administration than in the group with low dose of curdione administration(P﹤0.05);compared with the model group,the thrombus weight/weight-length ratio,the thrombus mass and NF-κB protein expression in the curdione dosing group were decreased.Conclusion Curdione can inhibit the formation of DVT in rats by influencing the fibrinolytic regulators.

关 键 词：莪术二酮 深静脉血栓 纤溶 抗血栓 D-二聚体 

分 类 号：R285[医药卫生—中药学]