艾灸通过调控α-黑色素细胞刺激素减轻膝骨关节炎大鼠炎症损伤  

Moxibustion Alleviates Inflammatory Injury in Rats with Knee Osteoarthritis by Regulatingα-Melanocyte Stimulating Hormone

作　　者：曾澜 汪雅璐 吴欣[1] 吴生兵[2,3,4] 周美启 ZENG Lan;WANG Yalu;WU Xin;WU Shengbing;ZHOU Meiqi(College of Acupuncture and Moxibustion,Anhui University of Chinese Medicine,Anhui Hefei 230012,China;Institute of Xin an Medicine and Traditional Chinese Medicine Modernization,Institute of Health and Medicine,Hefei Comprehensive National Science Center,Anhui University of Chinese Medicine,Anhui Hefei 230012,China;Anhui Province Key Laboratory of Meridian Viscera Correlationship,Anhui Hefei 230038,China;Institute of Acupuncture and Meridian,Anhui Academy of Chinese Medicine,Anhui Hefei 230038,China)

机构地区：[1]安徽中医药大学针灸推拿学院,安徽合肥230012 [2]安徽中医药大学合肥综合性国家科学中心大健康研究院新安医学与中医药现代化研究所,安徽合肥230012 [3]经脉脏腑相关安徽省重点实验室,安徽合肥230038 [4]安徽省中医药科学院针灸经络研究所,安徽合肥230038

出　　处：《安徽中医药大学学报》2024年第6期33-38,共6页Journal of Anhui University of Chinese Medicine

基　　金：合肥综合性国家科学中心大健康研究院新安医学与中医药现代化研究所专项资金资助(2023CXMMTCM010);安徽省高等学校科学研究项目(2023AH040113)。

摘　　要：目的从调控血清和关节液中α-黑色素细胞刺激素(α-melanocyte stimulating hormone,α-MSH)表达角度探究艾灸治疗膝骨关节炎(knee osteoarthritis,KOA)炎症损伤的机制。方法将SD大鼠随机分为正常组、模型组、艾灸组、α-ΜSH拮抗剂联合艾灸组,每组10只。采用碘乙酸钠注射法复制KOA大鼠模型。艾灸组、α-MSH拮抗剂联合艾灸组大鼠给予“犊鼻”“足三里”悬灸,每次每穴15 min,每日1次,持续4周,且α-MSH拮抗剂联合艾灸组大鼠于悬灸前给予α-MSH拮抗剂腹腔注射。治疗结束后采用苏木精—伊红染色法观察大鼠膝关节病理变化,ELISA法检测大鼠血清和关节液中α-MSH,血清肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)表达水平,Western blot法检测软骨组织中核因子κB抑制蛋白-α(inhibitor of NF-κB-α,IKB-α)和磷酸化p65(phosphorylated p65,p-p65)水平,实时荧光定量PCR法检测软骨组织IKB-α、p65 mRNA表达水平。结果艾灸能显著升高KOA大鼠血清和关节液中α-MSH水平以及软骨组织中IKB-α蛋白及其mRNA表达水平(P<0.05),显著降低KOA大鼠血清TNF-α、IL-1β水平以及软骨组织中p-p65蛋白和p65 mRNA表达水平(P<0.05);α-MSH拮抗剂能拮抗艾灸升高KOA大鼠软骨组织中IKB-α蛋白及其mRNA表达水平,并拮抗艾灸降低KOA大鼠软骨组织中p-p65蛋白和p65 mRNA表达水平。结论艾灸可以调控α-MSH的表达,减轻KOA大鼠炎症损伤,其作用机制可能与抑制NF-κB信号通路有关。Objective To investigate the therapeutic mechanism of moxibustion on inflammatory injury in knee osteoarthritis(KOA)by regulatingα-melanocyte stimulating hormone(α-MSH)in serum and synovial fluid.Methods Sprague-Dawley rats were randomly divided into normal group,model group,moxibustion group,andα-ΜSH antagonist+moxibustion group,with 10 rats in each group.Sodium iodoacetate injection was used to establish a rat model of KOA.The rats in the moxibustion group and theα-MSH antagonist+moxibustion group were treated with suspended moxibustion at Dubi and Zusanli for 15 minutes at each point,once a day for 4 weeks,and those in theα-MSH antagonist+moxibustion group were given intraperitoneal injection ofα-MSH antagonist before suspended moxibustion.After treatment,HE staining was used to observe the pathological changes of rat knee joints;ELISA was used to measure the expression level ofα-MSH in serum and synovial fluid and the expression levels of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)in serum;Western blot was used to measure the levels of inhibitor of NF-κB-α(IKB-α)and phosphorylated p65 in cartilage tissue,and quantitative real-time PCR was used to measure the mRNA expression levels of IKB-αand p65 in cartilage tissue.Results For the rats with KOA,moxibustion significantly increased the expression level ofα-MSH in serum and synovial fluid and the protein and mRNA expression levels of IKB-αin cartilage tissue(P<0.05),and it significantly reduced the serum levels of TNF-αand IL-1βand the protein expression level of phosphorylated p65 and the mRNA expression level of p65 in cartilage tissue(P<0.05).Theα-MSH antagonist antagonized the effect of moxibustion in upregulating the protein and mRNA expression levels of IKB-αin cartilage tissue and downregulating the protein expression level of phosphorylated p65 and the mRNA expression level of p65 in cartilage tissue.Conclusion Moxibustion can alleviate inflammatory injury in KOA rats by regulating the expression ofα-MSH,possibly throu

关 键 词：膝骨关节炎 艾灸 炎症损伤 Α-MSH NF-κB 

分 类 号：R246[医药卫生—针灸推拿学] R684.3[医药卫生—中医临床基础]