电针对糖尿病认知减退大鼠海马突触相关蛋白与突触结构的影响  

Effect of Electroacupuncture on Synapse-related Proteins and Synapse Structure in the Hippocampus of Rats with Diabetic Cognitive Impairment

作　　者：钱俊 袁爱红[2,3] 杨骏 付勤[1] 叶敏 张乐乐 樊吟秋 QIAN Jun;YUAN Aihong;YANG Jun;FU Qin;YE Min;ZHANG Lele;FAN Yinqiu(The First Clinical Medical College of Anhui University of Chinese Medicine,Anhui Hefei 230031,China;The First Affiliated Hospital of Anhui University of Chinese Medicine,Anhui Hefei 230031,China;Anhui Province Key Laboratory of Meridian Viscera Correlationship,Anhui Hefei 230031,China)

机构地区：[1]安徽中医药大学第一临床医学院,安徽合肥230031 [2]安徽中医药大学第一附属医院,安徽合肥230031 [3]经脉脏腑相关安徽省重点实验室,安徽合肥230031

出　　处：《安徽中医药大学学报》2024年第6期55-61,共7页Journal of Anhui University of Chinese Medicine

基　　金：安徽省自然科学基金项目(2108085MH308);袁爱红安徽省名中医工作室项目(皖中医药发展秘[2023]23号);第五批全国中医临床优秀人才研修项目(国中医药人教函[2022]1号)。

摘　　要：目的通过观察电针对大鼠海马突触相关蛋白[突触素(synaptophysin,SYN)、生长相关蛋白-43(growth-associated protein-43,GAP-43)和神经体质素-65(neuroplastin-65,NP65)]的表达及突触结构的影响,探讨电针改善糖尿病认知减退(diabetic cognitive impairment,DCI)的机制。方法将SD大鼠随机分为空白组10只,高脂饲料组90只。高脂饲料组大鼠先进行糖尿病模型的复制,糖尿病模型复制成功9周后,再采用Morris水迷宫实验筛选DCI大鼠,然后将DCI大鼠随机分成电针组和模型组,每组10只。电针组大鼠给予“胰俞”“后三里”和“内庭”穴针刺治疗,其中“后三里”“内庭”穴再用电针治疗,每日1次,每次15 min,每周6 d,连续干预4周。其余两组大鼠仅予固定处理而不给予治疗。模型复制前后及电针干预后,记录各组大鼠的随机血糖值,采用Morris水迷宫检测大鼠学习记忆能力;最后一次电针干预后,采用苏木精—伊红染色观察大鼠海马齿状回区(dentate gyrus,DG)组织病理形态改变,透射电子显微镜观察海马DG区突触结构变化情况,Western blot法检测海马组织SYN、GAP-43、NP65蛋白表达水平,实时荧光定量PCR法检测海马SYN、GAP-43、NP65 mRNA表达水平。结果电针可显著降低DCI大鼠随机血糖和逃避潜伏期(P<0.05),改善海马DG区神经元形态,显著降低突触间隙宽度和升高突触后致密物厚度(P<0.05),显著升高海马组织中SYN、GAP-43、NP65蛋白及其mRNA的表达水平(P<0.05)。结论电针可能通过调节突触相关蛋白SYN、GAP-43、NP65的表达及改善突触超微结构,修复神经元损伤,改善DCI大鼠认知功能。Objective To investigate the effect of electroacupuncture on the expression of synapse-related proteins[synaptophysin(SYN),growth-associated protein-43(GAP-43),and neuroplastin-65(NP65)]and synapse structure in the hippocampus of rats,as well as the mechanism of electroacupuncture improving diabetic cognitive impairment(DCI).Methods Sprague-Dawley rats were randomly divided into blank group with 10 rats and high-fat diet group with 90 rats.The rats in the high-fat diet group were used to establish a model of diabetes,and after 9 weeks of modeling,the Morris water maze test was used for the screening of DCI rats,which were then randomly divided into electroacupuncture group and model group,with 10 rats in each group.The rats in the electroacupuncture group were given acupuncture at"Yishu","Housanli"and"Neiting"and electroacupuncture at"Housanli"and"Neiting"for 15 minutes each time,once a day,6 days a week for 4 consecutive weeks,and the rats in the other two groups were fixed without any treatment.Random blood glucose level was recorded for each group before and after modeling and after electroacupuncture intervention,and the Morris water maze test was used to assess the learning and memory abilities of rats.After electroacupuncture intervention for the last time,HE staining was used to observe the pathological and morphological changes of the dentate gyrus(DG)region of the hippocampus;a transmission electron microscope was used to observe the changes in synapse structure in the DG region of the hippocampus;Western blot was used to measure the protein expression levels of SYN,GAP-43,and NP65 in hippocampal tissue,and RT-PCR was used to measure the mRNA expression levels of SYN,GAP-43,and NP65 in the hippocampus.Results Electroacupuncture significantly reduced random blood glucose level and escape latency in DCI rats(P<0.05)and improved the morphology of neuronal cells in the DG region of the hippocampus,and it also significantly reduced synaptic cleft width and increased the thickness of postsynaptic dense materia

关 键 词：糖尿病 认知减退 电针 突触结构 突触相关蛋白 学习记忆能力 

分 类 号：R245[医药卫生—针灸推拿学]