UPLC-Orbitrap-MS法快速分析鉴定紫芝醇溶性成分  

Rapid Analytical Identification of Ethanol-soluble Components of Ganoderma sinense Based on Ultra Performance Liquid Chromatography-Quadrupole/Electrostatic Field Orbitrap High Resolution Mass Spectrometry

作　　者：崩淑娟 李思雨 张静[1,2,3] 罗文慧 韩海伦 熊清平 彭灿 BENG Shujuan;LI Siyu;ZHANG Jing;LUO Wenhui;HAN Hailun;XIONG Qingping;PENG Can(Center for Xin an Medicine and Modernization of Traditional Chinese Medicine of IHM,Anhui University of Chinese Medicine,Anhui Hefei 230012,China;School of Pharmacy,Anhui University of Chinese Medicine,Anhui Hefei 230012,China;Generic Technology Research Center for Anhui TCM Industry,Anhui University of Chinese Medicine,Anhui Hefei230012,China;Huaiyin institute of technology,Jiangsu,Huaian 223001,China;Rural Revitalization Collaborative Technical Service Center of Anhui Province,Anhui University of Chinese Medicine,Anhui Hefei 230012,China;Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement,Anhui Hefei 230012,China)

机构地区：[1]安徽中医药大学新安医学与中医药现代化研究所,安徽合肥230012 [2]安徽中医药大学药学院,安徽合肥230012 [3]安徽中医药大学安徽省现代中药产业共性技术研究中心,安徽合肥230012 [4]淮阴工学院,江苏淮安223001 [5]安徽中医药大学安徽省乡村振兴协同技术服务中心,安徽合肥230012 [6]省部共建安徽道地中药材品质提升协同创新中心,安徽合肥230012

出　　处：《安徽中医药大学学报》2024年第6期94-100,共7页Journal of Anhui University of Chinese Medicine

基　　金：大健康研究院新安医学与中医药现代化研究所专项资金(2023CXMMTCM006);安徽省高校杰出青年科研项目(2023AH020035);安徽高校协同创新项目(GXXT-2022-075,GXXT-2022-084)。

摘　　要：目的采用超高效液相色谱—四极杆—静电场轨道阱高分辨质谱系统对紫芝的醇溶性成分进行快速识别并分析鉴定。方法采用Waters ACQUITY UPLC BEH C18色谱柱(1.7μm,150 mm×2.1 mm),以A(0.1%甲酸水)—B(乙腈)为流动相进行梯度洗脱,柱温为35℃,流速为0.2 mL/min,进样容积为3μL。在负离子模式下采集紫芝醇溶性的质谱数据,根据一级质谱的精确相对分子质量、二级质谱特征碎片,结合文献分析紫芝的醇溶性成分。结果通过Compound Discover 3.3软件、文献参考和人工校对的方法对紫芝醇提物共初步鉴定了16种成分。其中12种三萜类成分,4个脂肪酸类成分。结论所建立的方法完整、便捷,可为紫芝的药效物质基础差异及其作用机制研究提供一定的理论依据,并为紫芝成分鉴定及其质量控制标准提供理论支撑。Objective To perform rapid identification and analytical identification of the ethanol-soluble components of Ganoderma sinense by using ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry(UPLC-Orbitrap Exploris 120).Methods UPLC was performed on a Waters ACQUITY UPLC BEH C 18 column(1.7μm,150mm×2.1 mm)with A(0.1%formic acid water)-B(acetonitrile)as the mobile phase for gradient elution at a column temperature of 35℃,a flow rate of 0.2 mL/min,and an injection volume of 3μL.The mass spectrometry data of ethanol-soluble components were collected in the negative ion mode,and the ethanol-soluble components of Ganoderma sinense were analyzed based on the precise relative molecular mass of primary mass spectrometry,the characteristic fragments of secondary mass spectrometry,and related articles.Results In this experiment,a total of 16 components were preliminarily identified in the alcohol extract of the fruiting body of Ganoderma sinense by Compound Discover 3.3 software,literature review,and manual proofreading,among which there were 12 triterpenes and 4 fatty acids.Conclusion A relatively complete and convenient method is established to explore the differences in the material basis of Ganoderma sinense,which provides a certain theoretical basis for further research on the differences in the pharmacodynamic material basis and its mechanism of action and theoretical support for identifying the components of Ganoderma lucidum and establishing quality control standards.

关 键 词：紫芝 超高效液相色谱—四极杆—静电场轨道阱高分辨质谱 醇溶性成分 三萜类 

分 类 号：R284.1[医药卫生—中药学]