Advances in FRET-based biosensors from donor-acceptor design to applications  

作　　者：Wen-Chao Yang Shuang-Yu Li Shengnan Ni Guozhen Liu 

机构地区：[1]National Key Laboratory of Green Pesticide,Key Laboratory of Green Pesticide and Agricultural Bioengineering,Ministry of Education,Center for R&D of Fine Chemicals of Guizhou University,Guiyang,People’s Republic of China [2]CUHK(SZ)-Boyalife Joint Laboratory of Regenerative Medicine Engineering,Biomedical Engineering Programme,School of Medicine,The Chinese University of Hong Kong,Shenzhen,China [3]Ciechanover Institute of Precision and Regenerative Medicine,School of Medicine,The Chinese University of Hong Kong,Shenzhen,China

出　　处：《Aggregate》2024年第2期17-38,共22页聚集体（英文）

基　　金：National Natural Science Foundation of China,Grant/Award Numbers:U20A2038,32372584,22174121,22211530067,T2250710180;2022 Natural Science Foundation of Guangdong Provincial Basic and Applied Basic Research Fund(Guangdong Hybribio),Guangdong Peral River Talent Program,Grant/Award Number:2021CX02Y066;Shenzhen Bay Open Laboratory Fund 2021 by Shenzhen Bay Laboratory,CUHK(SZ)-Boyalife Joint Laboratory Fund,CUHK(SZ)-The Second Affiliated Hospital Joint Fund,Grant/Award Number:YXLH2205;University Development Fund,Grant/Award Number:UDF01002012。

摘　　要：Fluorogenic biosensors are essential tools widely used in biomedicine,chemical biology,environmental protection and food safety.Fluorescence resonance energy transfer(FRET)is a crucial technique for developing fluorogenic biosensors that provide mechanistic insight into bioprocesses through time-spatial bioimaging in living cells and organisms.Although extensive FRET-based sensors have been developed for detecting or imaging analytes of interest over the past decade,few comprehensive reviews have summarized the recent studies from the fundamental chemical angle about the design and application.In this work,the recent advance in the discovery of FRET biosensors using donor-acceptor dye combinations is described and they are classified based on different types of analytes,such as mall molecules,proteins,enzymes,nucleic acids and metal ions.This review provides molecular-level inspiration for the design of FRET-based biosensors,aiding in their application in biosensing and bioimaging.

关 键 词：BIOIMAGING donor-acceptor pairs fluorescence resonance energy transfer fluorogenic biosensors NANOMATERIALS 

分 类 号：O63[理学—高分子化学]