Matthew effect:General design strategy of ultra-fluorogenic nanoprobes with amplified dark–bright states in aggregates  

作　　者：Shinsuke Segawa Xinwen Ou Tianruo Shen Tomohiro Ryu Yuki Ishii Herman H.Y.Sung Ian D.Williams Ryan T.K.Kwok Ken Onda Kiyoshi Miyata Xuewen He Xiaogang Liu Ben Zhong Tang 

机构地区：[1]Translational and Advanced Bioimaging Laboratory,Department of Chemical and Biological Engineering,School of Engineering,The Hong Kong University of Science and Technology,Kowloon,Hong Kong,China [2]Department of Chemistry,Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction and Institute for Advanced Study,The Hong Kong University of Science and Technology,Kowloon,Hong Kong,China [3]Science,Mathematics and Technology Cluster,Singapore University of Technology and Design,Singapore,Singapore [4]Department of Chemistry,Graduate School of Science,Kyushu University,Nishi-ku,Fukuoka,Japan [5]The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou,College of Chemistry,Chemical Engineering and Materials Science,Soochow University,Suzhou,Jiangsu,China [6]School of Science and Engineering,Shenzhen Institute of Aggregate Science and Technology,The Chinese University of Hong Kong,Shenzhen,Guangdong,China

出　　处：《Aggregate》2024年第2期482-492,共11页聚集体（英文）

基　　金：Hong Kong PhD Fellowship Scheme,Grant/Award Number:PF18-15484;National Natural Science Foundation of China,Grant/Award Numbers:21788102,22274106;Research Grants Council of Hong Kong,Grant/Award Numbers:16306620,16303221,N_HKUST609/19,C6014-20W;Innovation and Technology Commission,Grant/Award Number:ITC-CNERC14SC01;Shenzhen Science and Technology Innovation Committee;JSPS KAKENHI,Grant/Award Numbers:JP23H01977,JP23H04631;JST the establishment of university fellowships towards the creation of science technology innovation,Grant/Award Number:JPMJFS2132。

摘　　要：Fluorescence imaging,a key technique in biological research,frequently utilizes fluorogenic probes for precise imaging in living systems.Tetrazine is an effective emission quencher in fluorogenic probe designs,which can be selectively damaged upon bioorthogonal click reactions,leading to considerable emission enhancement.Despite significant efforts to increase the emission enhancement ratio(I_(AC)/I_(BC))of tetrazine-functionalized fluorogenic probes,the influence of molecular aggregation on the emission properties has been largely overlooked in these probe designs.In this study,we reveal that an ultrahigh I_(AC)/I_(BC)can be realized in the aggregate system when tetrazine is paired with aggregation-induced emission(AIE)luminogens.Tetrazine amplifies its quenching efficiency upon aggregation and drastically reduce background emissions.Subsequent click reactions damage tetrazine and trigger significant AIE,leading to considerably enhanced I_(AC)/I_(BC).We further showcase the capability of these ultra-fluorogenic systems in selective imaging of multiple organelles in living cells.We term this unique fluorogenicity of AIE luminogen-quencher complexes with amplified dark-bright states as“Matthew effect”in aggregate emission,potentially providing a universal approach to attain ultrahigh I_(AC)/I_(BC)in diverse fluorogenic systems.

关 键 词：aggregation-induced emission bioorthogonal chemistry click chemistry fluorescence imaging TETRAZINE 

分 类 号：O62[理学—有机化学]