Medical ozone alleviates acute lung injury by enhancing phagocytosis targeting NETs via AMPK/SR-A1 axis  

作　　者：Chenxiao Yan Yong Zhang Lai Jin Xiaojie Liu Xuexian Zhu Qifeng Li Yu Wang Liang Hu Xueming He Hongguang Bao Xia Zhu Qian Wang Wen-Tao Liu 

机构地区：[1]The Key Laboratory of Rare Metabolic Disease,Department of Biochemistry and Molecular Biology,the Key Laboratory of Human Functional Genomics of Jiangsu Province,Key Laboratory of Targeted Intervention of Cardiovascular Disease,Collaborative Innovation Center for Cardiovascular Disease Translational Medicine,Nanjing Medical University,Nanjing,Jiangsu 211166,China [2]Department of Anesthesiology,Nanjing First Hospital,Nanjing Medical University,Nanjing,Jiangsu 210006,China [3]Jiangsu Key Laboratory of Neurodegeneration,Department of Pharmacology,Nanjing Medical University,Nanjing,Jiangsu 211166,China [4]Department of Anesthesiology,the Affiliated Drum Tower Hospital,Medical School of Nanjing University,Nanjing,Jiangsu 210009,China [5]Center for Clinical Research and Translational Medicine,Department of Anesthesiology,the Affiliated Lianyungang Oriental Hospital of Xuzhou Medical University,Lianyungang,Jiangsu 222042,China [6]Department of Anesthesiology,the Affiliated Lianyungang Oriental Hospital of Kangda College of Nanjing Medical University,Lianyungang,Jiangsu 222042,China

出　　处：《Journal of Biomedical Research》2024年第6期569-584,共16页生物医学研究杂志（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.82271252,82204542,and 81971047);the Lianyungang Science and Technology Program Project(Grant Nos.SF2122 and SF2214);the Scientific Research Project of Jiangsu Provincial Health Commission(Grant No.Z2021066);the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(Grant No.21KJB310019);the Open Project of Jiangsu Province Key Laboratory of Anesthesiology,Xuzhou Medical University(Grant No.XZSYSKF2021014).

摘　　要：Acute lung injury(ALI)linked to sepsis has a high mortality rate,with limited treatment options available.In recent studies,medical ozone has shown the potential to alleviate inflammation and infection.Here,we aimed to evaluate therapeutic potential of medical ozone in a mouse model of the sepsis-induced ALI by measuring behavioral assessments,lung function,and blood flow.Protein levels were quantified by Western blotting.In vitro,we performed experiments on bone marrow-derived macrophages(BMDMs)to investigate the effect of adenosine monophosphate(AMP)-activated protein kinase(AMPK)inhibitors and agonists on their phagocytic activity.The results showed that medical ozone significantly improved the survival rate,ameliorated lung injury,and enhanced lung function and limb microcirculation in mice with ALI.Notably,medical ozone inhibited the formation of neutrophil extracellular traps(NETs),a crucial factor in the ALI development.Additionally,medical ozone counteracted the elevated levels of tissue factor,matrix metalloproteinase-9,and interleukin-1β.In the ALI mice,the effects of ozone were abolished,and BMDMs showed an impaired capacity to engulf NETs following the Sr-a1 knockout.Under normal physiological conditions,the administration of an AMPK antagonist showed similar effects on the Sr-a1 knockout,significantly inhibiting the phagocytosis of NETs by BMDMs.In contrast,AMPK agonists enhanced this phagocytic process.In conclusion,medical ozone may alleviate the sepsis-induced lung injury through the AMPK/SR-A1 pathway,thereby enhancing the phagocytosis of NETs by macrophages.

关 键 词：SR-A1 NETS ALI PHAGOCYTOSIS ozone therapy 

分 类 号：R563[医药卫生—呼吸系统]