加味黄连温胆汤对胆汁反流性胃炎模型大鼠的治疗作用  

Therapeutic effect of modified Huanglian Wendan decoction on bile reflux gastritis model rats

作　　者：徐敏 赵鑫 程亚伟 那俸博 贾璐[2] 李艳梅[2] 王华宁 沈静 Xu Min;Zhao Xin;Cheng Yawei;Na Fengbo;Jia Lu;Li Yanmei;Wang Huaning;Shen Jing(Department of Spleen and Gastroenterology,the First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine,Kunming 650021;Southwest Forestry University,Kunming 650224,China)

机构地区：[1]云南中医药大学第一附属医院脾胃病科,昆明650021 [2]西南林业大学,昆明650224

出　　处：《解剖学杂志》2024年第5期389-392,397,共5页Chinese Journal of Anatomy

基　　金：云南省2020年高层次中医药人才培养对象项目(云卫中医发展中[2021]1号);云南省科技厅中医联合专项面上项目(202101AZ070001-071,202101AZ070001-254);云南省教育厅科学研究基金(2022Y340)。

摘　　要：目的:探讨加味黄连温胆汤对胆汁反流性胃炎(BRG)模型大鼠胃肠动力、胃黏膜屏障及其相关脑肠肽的影响。方法:将大鼠随机分为空白组、模型组、加味黄连温胆汤组、铝碳酸镁片组4组,除空白组外均制备BRG模型,加味黄连温胆汤组和铝碳酸镁片组于制备BRG模型第6周开始给药。观察各组大鼠的一般情况,计算胃排空率、小肠推进率,阿利新蓝-过碘酸雪夫染色观察胃黏膜屏障,ELISA检测脑肠肽表达。结果:与空白组比较,模型组大鼠一般情况差,体质量显著降低、胃指数显著升高;胃黏膜无蓝紫色染色;胃肠动力显著降低,相关脑肠肽表达有显著变化。与模型组比较,加味黄连温胆汤组和铝碳酸镁片组大鼠一般情况有所改善,体质量升高、胃指数降低;胃黏膜显现部分蓝紫色染色;胃肠动力改善;相关脑肠肽部分表达。结论:加味黄连温胆汤可改善BRG大鼠的一般情况,改善胃肠动力和胃黏膜屏障损伤,调节相关脑肠肽表达水平。Objective:To investigate the effect of modified Huanglian Wendan decoction on gastrointestinal motility,gastric mucosal barrier,and related brain-gut peptides in bile reflux gastritis(BRG)model rats.Methods:Rats were randomly divided into four groups:the blank group,the model group,the modified Huanglian Wendan decoction group,and the aluminum magnesium carbonate tablet gr oup.Except for the blank group,BRG models were prepared for all groups.The modified Huanglian Wendan decoction group and the aluminum magnesium carbonate tablet group began treatment in the sixth week after the BRG model was established..The general conditions of rats in each group were observed.The gastric emptying rate and small intestinal propulsion rate were calculated.The gastric mucosal barrier was observed by alcian blue periodic acid Schiff staining,and the expression of braingut peptide was detected by ELISA.Results:Compared with the control group,the general condition of the rats in the model group was poor.Their body weight was significantly reduced and their gastric index was significantly increased.There was no blue-purple staining in the gastric mucosa.Gastrointestinal motility was significantly reduced and the expression of related brain-gut peptides was significantly changed.Compared with the model group,the general conditions of the rats in the two treatment groups were improved,with increased body weight and decreased gastric index.Some blue-purple staining was seen in the gastric mucosa.Gastrointestinal motility was improved and related brain-gut peptides were partially expressed.Conclusion:The modified Huanglian Wendan decoction can improve the general condition of BRG rats,improve gastrointestinal motility and gastric mucosal barrier damage,and regulate the expression levels of related brain-gut peptid es.

关 键 词：加味黄连温胆汤 胆汁反流性胃炎 胃肠动力 胃黏膜屏障 脑肠肽 大鼠 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]