槐耳多糖调节Rho/ROCK信号通路对结肠癌SW620细胞恶性生物学行为的影响  

Impacts of Huaier polysaccharide on the malignant biological behavior of SW620 cells of colon cancer by regulating the Rho/ROCK signaling pathway

作　　者：温秀英 陈战 黄军利 陈洪标 林茜 温志坚 Wen Xiuying;Chen Zhan;Huang Junli;Chen Hongbiao;Lin Qian;Wen Zhijian(Department of General Surgery,Army 73rd Group Military Hospital,Xiamen 361000,China)

机构地区：[1]陆军第七十三集团军医院普通外科,厦门361000

出　　处：《解剖学杂志》2024年第5期410-414,429,共6页Chinese Journal of Anatomy

基　　金：厦门市医疗卫生指导性项目(3502Z20214ZD1187)。

摘　　要：目的:探讨槐耳多糖调节Rho/Rho相关卷曲螺旋蛋白激酶(ROCK)信号通路影响结肠癌SW620细胞恶性生物学行为。方法:人结肠癌细胞株SW620分为对照组、槐耳多糖组、水仙环素(Rho/ROCK途径激活剂)组、槐耳多糖+水仙环素组。分析各组细胞增殖、迁移、侵袭、凋亡和集落形成能力。免疫印迹检测RhoA、ROCK1、增殖细胞周期相关核抗原(Ki67)、基质金属蛋白酶2(MMP2)、caspase-3蛋白表达水平。建立裸鼠结肠癌移植瘤模型验证槐耳多糖对裸鼠体内移植瘤生长的影响。结果:槐耳多糖抑制SW620细胞增殖、迁移、侵袭、克隆集落数,并增加细胞凋亡以及RhoA、ROCK1、Ki67、MMP2蛋白表达水平。水仙环素逆转了槐耳多糖对SW620细胞恶性生物学行为的抑制作用。结论:槐耳多糖通过抑制Rho/ROCK信号通路抑制SW620细胞恶性生物学行为。Objective:To investigate the impacts of Huaier(trametes robiniophila murr)polysaccharide on the malignant biological behavior of SW620 cells of colon cancer by regulating Rho/Rho-associated coiled-coil kinase(ROCK)signaling pathway.Methods:Human colon cancer cell line SW620 was divided into control group,Huaier polysaccharide group,narciclasine(Rho/ROCK pathway activator)group,and Huaier polysaccharide+narciclasine group.Cell proliferation,migration,invasion,apoptosis,and colony-forming ability of the cells in each group were analyzed.The expression levels of RhoA,ROCK1,nuclear associated antigen(Ki67),matrix metalloproteinase 2(MMP2),and caspase-3 proteins were detected by Western blotting.The model of colon cancer was established in nude mice to verify the effect of Huaier polysaccharide on the growth of transplanted colon cancer in nude mice.Results:Huaier polysaccharide inhibited SW620 cell proliferation,migration,invasion,and colony formation and increased apoptosis as well as RhoA,ROCK1,Ki67,and MMP2 protein expression levels.Narcocycline reversed the inhibitory effect of Huaier polysaccharide on the malignant biological behavior of SW620 cells.Conclusion:Huaier polysaccharide inhibits the malignant biological behavior of SW620 cells by inhibiting the Rho/ROCK signaling pathway.

关 键 词：槐耳多糖 RHO/ROCK信号通路 结肠癌细胞 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]