远隔缺血预适应血浆外泌体增加血管内皮细胞低氧耐受  

Remote ischemic preconditioning with plasma exosomes increases vascular endothelial hypoxia tolerance

作　　者：张晰 杨文杰 邵国[2,3,4] Zhang Xi;Yang Wenjie;Shao Guo(College of Basic Medicine and Forensic Medicine,Baotou Medical College of Inner Mongolia University of Science and Technology,Baotou 014060;College of Medical Technology and Anesthesiology,Baotou Medical College of Inner Mongolia University of Science and Technology,Baotou 014060;Beijing Key Laboratory of Hypoxic Conditioning Translational Medicine,Xuanwu Hospital,Capital Medical University,Beijing 100069;Center for Translational Medicine and Department of Laboratory Medicine,The Third People′s Hospital of Longgang District,Shenzhen,Shenzhen 518112)

机构地区：[1]内蒙古科技大学包头医学院基础医学与法医学院,包头014060 [2]内蒙古科技大学包头医学院医学技术与麻醉学院,包头014060 [3]首都医科大学宣武医院低氧适应转化医学北京重点实验室,北京100069 [4]深圳市龙岗区第三人民医院转化医学中心,深圳518112

出　　处：《安徽医科大学学报》2024年第10期1809-1814,共6页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金项目(编号:82060337);深圳市龙岗区医疗卫生科技计划项目(编号:LGKCYLWS2021000033、LGKCYLWS2023025);深圳市科技计划项目(编号:JCYJ20220531092412028、JCYJ20230807121306012)。

摘　　要：目的研究远隔缺血预适应(RIPC)治疗前后卒中患者及健康对照血浆外泌体对人微血管内皮细胞(HMEC-1)低氧耐受的影响。方法HMEC-1分为空白对照组(C组)、氧-糖剥夺(OGD)处理组(OGD组),分别使用卒中患者RIPC前后(S-b组,S-a组)及健康成人RIPC前后(C-b组,C-a组)的血浆外泌体(35μg/ml)孵育HMEC-1细胞24 h,随后各组均给予OGD处理6 h,再灌注处理24 h。水溶性四氮唑(WST-1)法检测内皮细胞活力,测定caspase-3酶活力,Western blot检测紧密连接蛋白ZO-1、claudin-5表达水平。结果健康成人RIPC后血浆外泌体显著增加HMEC-1细胞活力,降低HMEC-1细胞caspase-3酶活性。结论RIPC治疗后血浆外泌体减少内皮细胞凋亡,增加内皮细胞低氧耐受。Objective To study the effect of plasma exosomes on hypoxia tolerance of human microvascular endothelial cells(HMEC-1)in stroke patients and healthy controls before and after remote ischemic preconditioning(RIPC)treatment.Methods HMEC-1 cells were divided into a blank control group(Group C)and oxygen glucose deprivation(OGD)treatment group,using plasma exosomes from stroke patients before and after RIPC(S-b group,S-a group)and healthy adults before and after RIPC(C-b group,C-a group).HMEC-1 cells were incubated with a concentration of exosome(35μg/ml)for 24 hours,followed by OGD treatment for 6 hours and reperfu-sion treatment for 24 hours in all groups.Water-soluble tetrazolium salt-1(WST-1)method was used to detect endothelial cell viability,caspase-3 enzyme activity was measured,and Western blot was used to detect the expression levels of zone occluden-1(ZO-1)and claudin-5.Results After RIPC in healthy adults,plasma exosomes significantly increased the cell activity of HMEC-1 cells and decreased the activity of caspase-3 enzyme in HMEC-1 cells.Conclusion After RIPC treatment,plasma exosomes reduced endothelial cell apoptosis and increased endothelial cell hypoxia tolerance.

关 键 词：远隔缺血预适应 外泌体 内皮细胞 低氧耐受 紧密连接 

分 类 号：R34[医药卫生—基础医学]