胰腺星状细胞通过旁分泌效应促进胰腺癌细胞的PIK3C2A表达及生长  

Pancreatic stellate cells promote the PIK3C2A expression and growth of pancreatic cancer cells through paracrine effects

作　　者：岳展 甄可妙 崔皓哲 应万涛 Yue Zhan;Zhen Kemiao;Cui Haozhe;Ying Wantao(School of Basic Medical Sciences,Anhui Medical University,Hefei230032;State Key Laboratory of Medical Proteomics,Beijing Proteome Research Center,National Center for Protein Sciences(Beijing),Beijing Institute of Lifeomics,Beijing102206)

机构地区：[1]安徽医科大学基础医学院,合肥230032 [2]医学蛋白质全国重点实验室,北京蛋白质组研究中心,国家蛋白质科学中心(北京),军事科学院军事医学研究院生命组学研究所,北京102206

出　　处：《安徽医科大学学报》2024年第11期1919-1925,共7页Acta Universitatis Medicinalis Anhui

基　　金：国家重点研发计划项目(编号:2021YFA1301602)。

摘　　要：目的研究胰腺星状细胞(PSC)的分泌蛋白对胰腺导管腺癌细胞(PANC-1)的作用及调控机制。方法通过间接共培养的方式,收集PSC的条件培养基(CM),分别培养PANC-1细胞0、2、24 h。CCK-8检测不同刺激时段PANC-1细胞的增殖表型。运用蛋白质组学手段,分析PANC-1细胞在蛋白水平上发生的变化,并用Western blot验证变化最显著的蛋白。结果与对照组相比,经PSC来源的CM刺激后,PANC-1细胞增殖速度加快;蛋白质组学数据分析结果表明,PANC-1细胞在PSC的CM分别培养0、2、24 h后,代谢相关通路的蛋白表达不断升高;基于Western blot验证了PIK3C2A在PANC-1细胞中的升高趋势,表明PSC的CM可能通过上调PIK3C2A的表达从而促进PANC-1细胞的增殖。结论PSC的CM可能通过旁分泌效应上调PIK3C2A的表达从而促进PANC-1细胞的增殖,提升了肿瘤微环境中PSC与胰腺癌细胞之间的交互对话机制的认识。Objective To study the effect and regulatory mechanism of secreted proteins from PSC on pancreatic ductal adenocarcinoma cells(PANC-1).Methods Conditioned medium(CM)from pancreatic stellate cells(PSC)was collected through an indirect co-culture method,and PANC-1 cells were cultured separately with CM for 0,2,and 24 h.The proliferation phenotype of PANC-1 cells under different stimulation periods was detected using the CCK-8 assay.Proteomic analysis was performed to analyze the changes in protein levels of PANC-1 cells,and the most significant protein changes were validated using Western blot.Results Compared with the control group,the proliferation rate of PANC-1 cells increased after being stimulated by PSC derived CM;The results of proteomic analysis showed that the protein expression of metabolic pathways in PANC-1 cells increased continuously after being cultured in PSC CM for 0,2,and 24 h.Western blot analysis confirmed an increasing trend of PIK3C2A in PANC-1 cells,indicating that the CM from PSC might promote the proliferation of PANC-1 cells by upregulating the expression of PIK3C2A.Conclusion The CM of PSC may promote the proliferation of PANC-1 cells by upregulating the expression of PIK3C2A,which improves the understanding of the mechanism of interaction between PSCs and pancreatic cancer cells in the tumor microenvironment.

关 键 词：胰腺星状细胞 条件培养基 间接共培养 蛋白质组学 

分 类 号：Q735.9[生物学—分子生物学]