烯醇化酶和层黏连蛋白在细菌性阴道病模型小鼠阴道中的表达  

Expression of enolase and laminin in the vagina of bacterial vaginosis of mice

作　　者：张瑞[1] 吴益青 尹海旭 李昶[1] 马爱昕 王君竹 白会会 刘朝晖 廖秦平 Zhang Rui;Wu Yiqing;Yin Haixu;Li Chang;Ma Aixin;Wang Junzhu;Bai Huihui;Liu Zhaohui;Liao Qinping(Dept of Obestetrics and Gynecology,Peking University First Hospital,Beijing100034;Northwest Women′s and Childern′s Hospital,Xi′an710061;Big Data and Public Policy Laboratory,School of Government,Peking University,Beijing100091;Dept of Obestetrics and Gynecology,Beijing Obstetrics and Gynecology Hospital,Beijing100026;Dept of Obestetrics and Gynecology,Beijing Tsinghua Changgung Hospital,Beijing102218)

机构地区：[1]北京大学第一医院妇产科,北京100034 [2]西北妇女儿童医院,西安710061 [3]北京大学政府管理学院大数据与公共政策实验室,北京100091 [4]北京妇产医院妇产科,北京100026 [5]北京清华长庚医院妇产科,北京102218

出　　处：《安徽医科大学学报》2024年第11期1939-1943,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金项目(编号:81471420);教育部博士学科点专项科研基金新教师类课题(编号:20130001120051)。

摘　　要：目的研究加德纳菌感染的小鼠和未感染的小鼠阴道上皮组织中的烯醇化酶和层黏连蛋白的差异情况,探讨烯醇化酶和层黏连蛋白与细菌性阴道病的发病关系。方法用来自细菌性阴道病患者阴道分泌物中分离、纯化、鉴定的加德纳菌菌株,感染小鼠阴道,建立细菌性阴道病小鼠模型。将建模成功的小鼠和建模失败的小鼠分别定义为成功组和失败组。Western blot检测两组小鼠阴道上皮组织中的烯醇化酶和层黏连蛋白的表达。统计建模成功率,比较两组烯醇化酶和层黏连蛋白的表达差异。结果1株阴道加德纳菌菌株感染10只SPF级KM小鼠,7只小鼠符合细菌性阴道病诊断标准,3只建模失败,建模成功率70%。成功组的小鼠阴道上皮组织中的层黏连蛋白和烯醇化酶的含量明显高于失败组(P<0.05)。结论烯醇化酶和层黏连蛋白在加德纳菌感染的小鼠和未感染小鼠的表达存在明显差异,这提示烯醇化酶和层黏连蛋白可能参与了加德纳菌诱发的细菌性阴道病的发生。Objective To investigate the differences in enolase and laminin levels in vaginal epithelial tissues between mice successfully infected with Gardnerella and mice not infected with Gardnerella,providing information for further exploration of the correlation between enolase and laminin levels and the incidence of bacterial vaginosis.Methods Gardnerella strains isolated,purified,and identified from vaginal secretions of patients with bacterial vaginosis were used to infect the vagina of mice and establish a mouse model of bacterial vaginosis.Successful and failed mice was defined as successful and failed groups,respectively.Differential expression of enolase and laminin in the vaginal epithelial tissue of two groups of mice was detected by Western blot.Modeling success rate was statistically analyzed,and the expression differences of enolase and laminin was compared between two groups.Results One strain of Gardnerella vaginalis infected 10 SPF grade KM mice,7 mice met the diagnostic criteria for bacterial vaginosis,and 3 mice failed to model,with a success rate of 70%.Western blot was used to detect protein expression levels,and the levels of laminin and enolase in the successfully modeled mouse vaginal epithelial tissue were significantly higher than those in the failed modeling group,with statistical differences between the two groups(P<0.05).Conclusion Enolase and laminin may be involved in the occurrence of bacterial vaginosis,however,further research is needed to determine the mechanisms through which they trigger the occurrence and development of the disease.

关 键 词：动物模型 细菌性阴道病 烯醇化酶 层黏连蛋白 阴道加德纳菌 发病机制 

分 类 号：R711.31[医药卫生—妇产科学]