长期高血糖对1型糖尿病食蟹猴模型肾脏病理学的影响  

Effects of long-term hyperglycemia on renal pathology of type 1 diabetic mellitus cynomolgus monkeys

作　　者：况昕宇 姜佩文 吴冬君 邹春林 宋琼[1,2,3,4,5] Kuang Xinyu;Jiang Peiwen;Wu Dongjun;Zou Chunlin;Song Qiong(School of Preclinical Medicine,Guangxi Key Laboratory of Regenerative Medicine,Guangxi Medical University,Nanning530021;Center for Translational Medicine,Guangxi Key Laboratory of Regenerative Medicine,Guangxi Medical University,Nanning530021;Key Laboratory of Longevity and Ageing-Related Disease of Chinese Ministry of Education,Guangxi Key Laboratory of Regenerative Medicine,Guangxi Medical University,Nanning530021;Institute of Neuroscience and Guangxi Key Laboratory of Brain Science,Guangxi Key Laboratory of Regenerative Medicine,Guangxi Medical University,Nanning530021;Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed by the Province and Ministry,Guangxi Key Laboratory of Regenerative Medicine,Guangxi Medical University,Nanning530021)

机构地区：[1]广西医科大学基础医学院,南宁530021 [2]广西医科大学转化医学研究中心,南宁530021 [3]广西医科大学“长寿与老年相关疾病”教育部重点实验室,南宁530021 [4]广西医科大学神经科学研究所“广西脑科学研究”重点实验室,南宁530021 [5]广西医科大学再生医学与医用生物资源开发应用省部共建协同创新中心,广西再生医学重点实验室,南宁530021

出　　处：《安徽医科大学学报》2024年第11期1998-2003,共6页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金项目(编号:81670750、82160482);国家级大学生创新创业项目(编号:202310598012)。

摘　　要：目的通过建立链脲佐菌素(STZ)诱导的1型糖尿病(T1DM)食蟹猴模型,观察长期高血糖对T1DM食蟹猴肾脏病理学的影响。方法将8只4岁雄性食蟹猴,随机分为对照组和模型组。4只食蟹猴作为对照组,4只食蟹猴通过注射STZ诱导构建T1DM模型作为模型组。运用HE染色、PAS染色观察肾脏病理改变,透射电镜观察肾脏肾小球超微结构,采用形态学计量分析测算肾小球面积、肾小球基底膜厚度、足细胞足突的相对数量以及平均面积。结果与对照组相比,模型组肾脏病理学改变:肾小球面积增大(P<0.01)、基底膜增厚,毛细血管袢受压,内皮细胞窗孔排列紊乱,系膜细胞增生,系膜基质增多、糖蛋白积聚,足突数量减少(P<0.001),足突平均面积减少(P<0.05),足细胞裂孔增宽,融合足突数量增加(P<0.001)。结论长期高血糖可致T1DM食蟹猴模型肾脏病理学改变。Objective To investigate the effects of long-term hyperglycemia on renal pathology of type 1 diabetic mellitus(T1DM)cynomolgus monkeys by establishing streptozotocin(STZ)-induced T1DM.Methods Eight 4-year-old male cynomolgus monkeys were randomly divided into control and model groups.Four cynomolgus monkeys were used in the control group,and four cynomolgus monkeys were injected with streptozotocin to create a T1DM model.Hematoxylin-eosin(HE)staining and periodic acid-Schiff(PAS)staining were used to observe renal pathological changes.The histological characteristics and changes were observed under transmission electron microscope.Morphometric measurements were used to analyze the glomerular area,the thickness of the glomerular basement membrane,the proportion of glomerular podocyte foot processes and the average area of foot processes.Results In comparison to the control group,the model group showed pathological changes in the kidneys,including increased glomerular area(P<0.01),thickened basement membrane,capillary loop compression,disordered arrangement of endothelial cell fenestrations,proliferation of mesangial cells,mesangial expansion,accumulation of mesangial matrix glycoproteins,a decrease in the number of foot processes(P<0.001),a decrease in the average size of the foot processes(P<0.05),widened podocyte slit diaphragm,and an increase in the number of fused foot processes(P<0.001).Conclusion Cynomolgus monkeys with T1DM suffer from renal pathological changes due to long-term hyperglycemia.

关 键 词：链脲佐菌素 1型糖尿病 食蟹猴 肾脏 足细胞 病理学 

分 类 号：R587.1[医药卫生—内分泌]