光谱法研究Pb^(2+)和Cd^(2+)与海南木榄叶DNA的相互作用  

Study on the Interaction between Pb^(2+),Cd^(2+)and DNA of Hainan Bruguiera gymnorhiza Leaf by Spectroscopic Method

作　　者：陈婉慧 宋媛 陈光英[1] 何文英[1] CHEN Wanhui;SONG Yuan;CHEN Guangying;HE Wenying(Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education,Hainan Normal University,Haikou 571158,China)

机构地区：[1]海南师范大学热带药用资源化学教育部重点实验室,海南海口571158

出　　处：《海南师范大学学报（自然科学版）》2024年第3期313-319,共7页Journal of Hainan Normal University（Natural Science）

基　　金：国家自然科学基金项目(22177023);海南省科技计划重点研发项目(ZDYF2022XDNY197)。

摘　　要：木榄是一种具有较高经济价值的药用红树植物,对沿海地区的生态也有良好的保护作用。本研究利用紫外光谱和荧光光谱法对2种重金属离子Pb^(2+)和Cd^(2+)与海南木榄叶DNA的相互作用进行了研究。结果表明,Pb^(2+)和Cd^(2+)的存在对木榄叶DNA的结构有一定影响,且均以沟槽结合的模式与木榄叶DNA相互作用;热力学参数表明Pb^(2+)与木榄叶DNA可自发反应,氢键和范德华力为主要的相互作用力,而Cd^(2+)与DNA为非自发反应,其相互作用力以疏水作用力为主。该研究可为从分子层面深入了解红树植物耐重金属离子的作用机制提供理论支持。Bruguiear gymnorrhiza is one kind of medicinal mangrove plants with higher commercial value,which also has good protective effect on the ecology of the coastal area.In this study,the interaction of two kinds of heavy metals including Pb^(2+),Cd^(2+)with the DNA extracted from the leafs of Hainan Bruguiear gymnorrhiza were investigated by using UV-vis and fluorescence spectroscopes.The results indicated that the presence of Pb^(2+),Cd^(2+)had a certain influence on the structure of DNA and the interaction could occur in a groove-binding mode.The thermodynamic parameters showed that it is a sponta⁃neous reaction between Pb^(2+)and DNA with hydrogen bond and Vander Waals force as the main interaction forces.Howev⁃er,Cd^(2+)unspontaneously interacted with DNA and hydrophobic force played a main role in the binding process.This re⁃search can provide some theoretical support for further understanding the mechanism of mangrove plants'tolerance of heavy metal ions.

关 键 词：木榄叶 DNA 重金属离子 光谱法 相互作用 

分 类 号：R285[医药卫生—中药学]