解脲沙雷菌(Serratia ureilytica)次级代谢产物及其抗菌活性研究  

Study on the Secondary Metabolites and Antibacterial Activity of Serratia ureilytica

作　　者：魏艳梅 张东辉 莫土香 李鹏 陈光英[1,2] WEI Yanmei;ZHANG Donghui;MO Tuxiang;LI Peng;CHEN Guangying(Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education,Hainan Normal University,Haikou 571158,China;Key Laboratory of Tropical Medicinal Plant Chemistry of Hainan Province,College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou 571158,China;College of Life Sciences,Hainan Normal University,Haikou 571158,China)

机构地区：[1]海南师范大学热带药用资源化学教育部重点实验室,海南海口571158 [2]海南师范大学化学与化工学院,海南省热带药用植物化学重点实验室,海南海口571158 [3]海南师范大学生命科学学院,海南海口571158

出　　处：《海南师范大学学报（自然科学版）》2024年第3期331-337,共7页Journal of Hainan Normal University（Natural Science）

基　　金：海南省研究生创新科研课题(Qhyb2021-43);海南省院士创新平台科研专项基金(YSPTZX202030)。

摘　　要：本研究对一株海洋来源细菌(Serratia ureilytica)的次级代谢产物进行系统分离。采用多种柱色谱分离方法从该菌中分离得到16个单体化合物,运用NMR技术鉴定所得化合物分别为:环(色氨酸-丝氨酸)(1)、环(甘氨酸-色氨酸)(2)、环(苯丙氨酸-谷氨酰胺)(3)、环(亮氨酸-谷氨酰胺)(4)、环(L-酪氨酸-4-羟基-D-脯氨酸)(5)、环(L-苯丙氨酸-4-羟基-D-脯氨酸)(6)、环(L-苯丙氨酸-4-羟基-L-脯氨酸)(7)、环(L-4-羟基-脯氨酸-L-亮氨酸)(8)、环(脯氨酸-酪氨酸)(9)、环(D-脯氨酸-L-酪氨酸)(10)、环(S-脯氨酸-S-亮氨酸)(11)、环(L-脯氨酸-L-缬氨酸)(12)、环(酪氨酸-甘氨酸)(13)、环(R-苯丙氨酸-S-丝氨酸)(14)、环(L-苯丙氨酸-甘氨酸)(15)和环(甘氨酸-亮氨酸)(16)。化合物1~16均为首次从解脲沙雷菌中分离得到。抗菌活性显示,粗提物对青枯菌有显著的拮抗作用,而单体化合物对青枯菌以及5株人体致病菌均无明显活性。The secondary metabolites of marine bacteria(Serratia ureilytica)were studied systematically,and sixteen com⁃pounds were isolated by various chromatographic methods and the structures of these compounds were identified by 1D and 2D NMR.The compounds were identified to be cyclo-(L-Trp-L-Ser)(1),cyclo-(Gly-L-Trp)(2),cyclo-(Phe-Gln)(3),cyc⁃lo-(Leu-Gln)(4),cyclo-(L-tyrosyl-cis-4-hydroxy-D-proline)(5),cyclo-(L-phenylalanine-D-4-hydroxyproline)(6),cyc⁃lo-(L-4-OH-Pro-L-Phe)(7),cyclo-(L-4-Hyp-L-Leu)(8),cyclo-(L-Pro-L-Tyr)(9),cylco-(D-Pro-L-Tyr)(10),cyclo-(S-Pro-S-Leu)(11),cyclo-(L-Pro-L-Val)(12),cyclo-(Tyr-Gly)(13),cyclo-(R-Phe-S-Ser)(14),cyclo-(L-Phe-Gly)(15),and cyclo-(Gly-Leu)(16)respectively.All the compounds were isolated from the S.ureilytica for the first time.The antibac⁃terial activity showed that the crude extract had significant antagonistic effect on Ralstonia solanacearum,while,no com⁃pound showed antibacterial activity against Ralstonia solanacearum and 5 strains of human pathogenic bacteria.

关 键 词：解脲沙雷菌 次级代谢产物 抗菌活性 

分 类 号：R284.1[医药卫生—中药学]