常用消毒剂对3种人畜共患病病原菌的杀灭效果观察  

Observation on the killing effect of common used disinfectants against three kinds of zoonotic pathogens

作　　者：李军丽 黄洋[1] 王劲松[1] 赵爱华[1] 陈国庆[1] LI Jun-li;HUANG Yang;WANG Jin-song;ZHAO Ai-hua;CHEN Guo-qing(National Institutes for Food and Drug Control&National Medical Products Administration(NMPA)Key Laboratory for Quality Research and Evaluation of Biological Products&National Health Commission(NHC)Key Laboratory of Research on Quality and Standardization of Biotech Products,Beijing 102629,China)

机构地区：[1]中国食品药品检定研究院国家药品监督管理局生物制品质量研究与评价重点实验室国家卫生健康委员会生物技术产品检定方法及其标准化重点实验,北京102629

出　　处：《中国消毒学杂志》2024年第11期801-804,共4页Chinese Journal of Disinfection

基　　金：北京市自然科学基金(L242121)。

摘　　要：目的 比较9种常用消毒剂对减毒牛分枝杆菌、炭疽杆菌弱毒株芽孢和布鲁菌弱毒株的杀灭效果。方法采用悬液定性杀菌试验,观察84消毒液、碘伏、二溴海因、过氧化氢溶液、乙醇、石碳酸、氯己定、苯扎溴铵和戊二醛分别对3种细菌作用5和45 min的杀灭效果,采用细菌培养进行效果评价。结果 0.55%84消毒液、0.5%碘伏、3%过氧化氢溶液、75%乙醇和2%戊二醛等消毒液作用5 min能有效杀灭减毒牛分枝杆菌,而0.1%二溴海因和0.3%苯扎溴铵作用时间延长至45 min,仍无明显杀灭效果。0.55%84消毒液、0.5%碘伏、3%过氧化氢溶液须进行长时间浸泡对炭疽杆菌弱毒株芽孢有一定的杀灭效果。75%乙醇、5%石碳酸、0.2%氯己定和0.3%苯扎溴铵等作用45 min无法完全杀灭炭疽杆菌弱毒株芽孢。结论 75%乙醇和0.55%84消毒液对减毒牛分枝杆菌和布鲁菌弱毒株均有较好的杀灭效果,而对炭疽杆菌弱毒株芽孢则无明显的杀灭效果。Objective To compare the killing effects of nine commonly used disinfectants on attenuated Mycobacterium bovis,Bacillus anthracis attenuated strains spores and Brucella spp attenuated strains.Methods The suspension qualitative bactericidal test was used to detect the effect of 84 disinfectant,0.5% iodophor,50% dibromohydantoin,30% hydrogen peroxide solution,75% ethanol,50% carbolic acid,0.2% chlorhexidine,3% benzalkonium bromide and 2% glutaraldehydee on three bacteria for 5 and 45 min,respectively.Bacterial culture method was used to evaluate the killing effect of each disinfectant.Results 0.55% 84 disinfectant,0.5% iodophor,3% hydrogen peroxide solution,75% ethanol and 2% glutaraldehyde could effectively kill attenuated Mycobacterium bovis after 5 min,while 0.1% dibromohain and 0.3% benzalammonium bromide had no obvious killing effect even if time was extended to 45 min.Similarly,0.55% 84 disinfectant solution,0.5% iodophor and 3%hydrogen peroxide solution had a certain efficacy on Bacillus anthracis attenuated strains spores only when incubating for a long time.75% ethanol,5% carbonic acid,0.2% chlorhexidine and 0.3% benzalammonium bromide could not completely kill Bacillus anthracis spores incubating for 45 min.Conclusions 75% ethanol and 0.55% 84 disinfectant have good killing effect on attenuated Mycobacterium bovis and Brucella spp attenuated strains,but have no obvious killing effect on Bacillus anthracis attenuated strain spores.

关 键 词：消毒剂 结核分枝杆菌 炭疽杆菌弱毒株芽孢 布鲁菌 悬液定性杀菌试验 

分 类 号：R187.2[医药卫生—流行病学]