Effects and driving factors of domestic sewage from different sources on nitrous oxide emissions in a bog  

不同来源生活污水对泥炭沼泽湿地N_(2)O排放的影响及其驱动因素

作　　者：Yue Li Zhongbing Chen Xue Wang Haibo Jiang Chunguang He Yao Shi Lianxi Sheng 

机构地区：[1]State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration,School of Environment,Northeast Normal University,Changchun 130000,China [2]Key Laboratory for Vegetation Ecology,Ministry of Education,Northeast Normal University,Changchun 130000,China [3]Department of Applied Ecology,Faculty of Environmental Sciences,Czech University of Life Sciences Prague,Praha-Suchdol 16500,Czech Republic [4]College of Engineering,Jilin Normal University,Siping 136000,China

出　　处：《Journal of Plant Ecology》2024年第5期52-64,共13页植物生态学报（英文版）

基　　金：supported by the National Key R&D Program of China(2022YFF1300900);the National Natural Science Foundation of China(32271624);the Foundation of Jilin Scientifc and Technological Development Project(20220203003SF);the Education Department of Jilin Province(JJKH20230516KJ).

摘　　要：Direct sewage discharge may enhance soil nitrous oxide(N_(2)O)emissions,worsening the greenhouse effect.However,the effects of sewage discharge into bogs on N_(2)O flux,drivers and influencing mechanisms remain unclear.Additionally,investigating the impact of reclaimed water on N_(2)O flux is important for bog replenishment and water shortage alleviation.This study simulated sewage from different sources into a bog and analyzed N_(2)O fluxes,soil(organic carbon,total nitrogen,ammonium nitrogen,nitrate nitrogen,total phosphorus,available phosphorus,pH and electrical conductivity),plant(species richness and biomass)and microorganisms(ammonia-oxidizing archaea,napA,nirS,nirK and nosZ genes).Results showed that the reclaimed water did not significantly change N_(2)O flux,while 50%tap water mixed with 50%domestic sewage and domestic sewage significantly increased the N_(2)O flux.Among soil factors,available nitrogen and pH were key in influencing N_(2)O flux.Among plant parameters,species richness was the primary factor affecting N_(2)O flux.Nitrogen transformation functional genes contributed the most to the increase in the N_(2)O fluxes,with an increase in domestic sewage input leading to a higher abundance of these genes and subsequent N_(2)O emissions.Therefore,domestic sewage should be considered,as it significantly increases N_(2)O emissions by affecting the soil,plants and microorganisms,thereby increasing the global warming potential.This study’s findings suggest that using treated reclaimed water for bog replenishment could be an environmentally friendly approach to wetland management.污水直接排放可能会增加土壤N_(2)O排放量,加剧温室效应。然而,污水排放到泥炭沼泽湿地对N_(2)O通量的影响、主要驱动因素和影响机制尚不清楚。此外,研究再生水对N_(2)O通量的影响对于泥炭沼泽湿地生态补水以及缓解水资源短缺具有重要意义。本研究通过模拟不同来源生活污水输入泥炭沼泽湿地的实验,测定了不同处理下N_(2)O排放通量、土壤理化性质(有机碳、全氮、铵态氮、硝态氮、全磷、速效磷、pH和电导率)、植物参数(物种丰富度和生物量)和土壤氮循环微生物关键功能基因丰度(AOA、napA、nirS、nirK和nosZ)。结果表明,再生水处理未显著改变泥炭沼泽湿地N_(2)O排放通量,50%的自来水混合50%的生活污水处理、生活污水处理显著增加了泥炭沼泽湿地N_(2)O排放通量。在土壤因子中,有效氮和pH是影响N_(2)O通量的关键因子;在植物参数中,物种丰富度是影响N_(2)O通量的重要因子;氮循环功能基因主导泥炭沼泽湿地N_(2)O排放,对N_(2)O排放通量增加的贡献最大。随着污水输入量的增加,AOA、napA、nirS、nirK和nosZ基因丰度的增加促进了硝化和反硝化过程,导致泥炭沼泽湿地N_(2)O排放通量增加。综上,生活污水会通过影响土壤、植物和微生物进而促进泥炭沼泽湿地N_(2)O排放,因而应被引起高度关注;处理过的再生水很可能是一种环境友好型的泥炭沼泽湿地补水水源。

关 键 词：SEWAGE BOG N_(2)O flux species richness nitrogen transformation functional microorganism 

分 类 号：Q94[生物学—植物学]