Cyanobacterial bloom affects structural and functional succession of microbial communities in urban wetland sediments in the Qinghai Plateau  

蓝藻水华影响高原城市湿地沉积物微生物群落结构和功能演替

作　　者：Yi Wu Xiaoyan Wei Xufeng Mao Xiuhua Song Xijie Wei Shunbang Xie Hongyan Yu Lele Zhang Yongxiao Yang 

机构地区：[1]Key Laboratory of Tibetan Plateau Land Surface Processes and Ecological Conservation(Ministry of Education),Qinghai Normal University,Xining 810001,China [2]Qinghai Province Key Laboratory of Physical Geography and Environmental Process,College of Geographical Science,Qinghai Normal University,Xining 810001,China [3]School of Economics and Management,Qinghai Normal University,Xining 810003,China [4]Management and Service Center of Huangshui National Wetland Park,Xining 810001,China [5]Academy of Animal and Veterinary Science,Qinghai University,Xining 810003,China [6]Qilian Mountain National Park Qinghai Province Management Bureau,Xining 810001,China

出　　处：《Journal of Plant Ecology》2024年第5期65-77,共13页植物生态学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(52070108);Project of Philosophy and Social Sciences of Qinghai Province(23ZCY042).

摘　　要：Algal blooms significantly affect microbial communities in wetland ecosystems.However,little is known about the succession of sediment microbial communities during algal blooms.This study aimed to investigate the temporal patterns of sediment bacterial community structure and function succession during algal blooms(March to May 2022)with high-throughput sequencing technology.To this end,algal blooms were divided into the bloom stage(BS),decomposition stage(DS)and end stage(ES).The results showed that:(i)The algal blooms were dominated by Microcystis species within Cyanobacteria.Both phytoplankton abundance and biomass reached the peak in the BS,with 45.78×105 cells/L and 5.97 mg/L,respectively.(ii)The alpha diversity indices of the sediment bacterial community showed a monotonically increasing trend,with the lowest value in the BS.Cyanobacterial blooms reduced the relative abundance of Actinobacteria and Chloroflexi,and raised that of Firmicutes,Proteobacteria and Bacteroidetes.(iii)Cyanobacterial blooms remarkably decreased the complexity of the co-occurrence network of the sediment bacterial community,which resisted extreme environmental conditions in the BS and DS by increasing positive relationships.(iv)Mantel test showed that phytoplankton biomass,T,dissolved oxygen and organic carbon had significant effects on the sediment bacterial community.The results of this study are important for the ecological management of algal blooms through microorganisms.水华暴发显著影响湿地生态系统微生物群落特征。然而,对水华暴发如何影响沉积物微生物群落演替的认识仍不清楚。本研究调查了青藏高原城市湿地水华生消过程中沉积物细菌群落演替的动态特征。根据水环境特征的变化情况,将水华划分为3个时期:暴发期、消亡期和结束期。研究结果表明,湿地水华以蓝藻门的微囊藻为优势种,其丰度和生物量均在暴发期达到峰值,分别为45.78×105 cells/L和5.97 mg/L。沉积物细菌群落多样性呈单调上升趋势。蓝藻水华降低了放线菌门和绿弯菌门的相对丰度,增加了变形菌门、拟杆菌门和厚壁菌门的相对丰度。此外,蓝藻水华显著降低了沉积物细菌群落互作网络的复杂度。曼特尔检验表明,浮游植物生物量、水温、溶解氧和有机碳是影响沉积物细菌群落演替的关键因素。上述研究结果对利用微生物进行水华生态治理具有重要启示。

关 键 词：algal blooms bacterial community EUTROPHICATION co-occurrence network FAPROTAX 

分 类 号：Q94[生物学—植物学]