RBP-J调控病理性Th17细胞在肝纤维化进展中的作用  

Effect of RBP-J regulating pathological Th17 cells in the progression of liver fibrosis

作　　者：徐硕 宋美瑛 蒋素丽 杜翘楚 刘贵先 张蓓[1] XU Shuo;SONG Meiying;JIANG Suli;DU Qiaochu;LIU Guixian;ZHANG Bei(Department of Immunology,School of Basic Medicine,Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学基础医学院免疫学系,山东青岛266071

出　　处：《青岛大学学报（医学版）》2024年第5期647-652,共6页Journal of Qingdao University(Medical Sciences)

基　　金：青岛市科技惠民资助项目(22-3-7-smjk-7-nsh)。

摘　　要：目的探讨重组信号结合蛋白Jκ(RBP-J)对病理性Th17(pTh17)细胞的调控以及RBP-J/pTh17轴在肝纤维化进展中的作用。方法收集5例正常人(供者)和5例肝纤维化病人(受者)的肝组织及外周血样本。应用实时荧光定量PCR(qRT-PCR)、免疫组织化学和Western blot方法,检测肝组织RBP-J和白细胞介素23受体(IL-23R)的表达,应用流式细胞术方法检测外周血中pTh17细胞的频率。结果与健康对照组相比,肝纤维化病人肝组织中RBP-J和IL-23R蛋白表达量以及IL-23R基因表达水平显著增加(t=5.70~83.34,P<0.05)。与健康对照组相比,肝纤维化病人外周血中pTh17细胞频率也明显增加(t=18.88,P<0.01)。结论肝损伤时,RBP-J可能通过驱动IL-23R表达促进pTh17细胞分化,从而促进肝纤维化的进程,提示RBP-J/pTh17轴可能是治疗肝纤维化的一个潜在靶点。Objective To explore the regulatory effect of recombinant signal-binding protein Jκ(RBP-J)on pathological T helper 17(pTh17)cells and the role of the RBP-J/pTh17 axis in the progression of liver fibrosis.Methods Liver tissues and peripheral blood samples from five normal individuals(donors)and five patients with liver fibrosis(recipients)were collected.Real-time fluorescence quantitative polymerase chain reaction,immunohistochemistry,and Western blot were applied to measure the expression levels of RBP-J and interleukin 23 receptor(IL-23R)in the liver tissues;flow cytometry was used to determine the frequency of pTh17 cells in peripheral blood samples.Results In the liver tissues of patients with liver fibrosis,the protein expression of RBP-J and IL-23R and the gene expression level of IL-23R were significantly increased in comparison with that of healthy controls(t=5.70-83.34,P<0.05).In the peripheral blood of patients with liver fibrosis,pTh17 cell frequency was also significantly increased compared with that of healthy controls(t=18.88,P<0.01).Conclusion In liver injury,RBP-J may promote pTh17 cell differentiation by driving IL-23R expression and thus promote the progression of liver fibrosis,suggesting that the RBP-J/pTh17 axis may be a potential target for the treatment of liver fibrosis.

关 键 词：免疫球蛋白J重组信号序列结合蛋白质 TH17细胞 肝纤维化 

分 类 号：R392.12[医药卫生—免疫学] R563.9[医药卫生—基础医学]