反复禁锢应激对小鼠mPFC脑区神经元突触活动影响  

Effect of repeated restraint stress on synaptic activity of pyramidal neurons in the medial prefrontal cortex of mice

作　　者：杜安琪 李楠[2] 王思涵 闫震 沈若武[1] 周宇[2] DU Anqi;LI Nan;WANG Sihan;YAN Zhen;SHEN Ruowu;ZHOU Yu(Department of Special Medicine,School of Basic Medicine,Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学基础医学院特种医学系,山东青岛266071 [2]青岛大学基础医学院生理学教研室,山东青岛266071

出　　处：《青岛大学学报（医学版）》2024年第5期680-683,共4页Journal of Qingdao University(Medical Sciences)

基　　金：山东省自然科学基金重大基础研究项目(ZR20-19ZD34)。

摘　　要：目的探讨反复禁锢应激对野生型C57BL6/J小鼠内侧前额叶皮质(mPFC)锥体神经元突触活动的影响。方法将12~20周龄雄性小鼠随机分为对照组和禁锢组。禁锢组给予反复禁锢应激刺激,每天1 h,连续4 d。采用高架十字迷宫实验评估小鼠的焦虑样行为,采用全细胞膜片钳技术记录小鼠mPFC脑区锥体神经元的兴奋性和抑制性突触后电流。结果禁锢组的小鼠焦虑样行为明显增加(t=3.069,P<0.01);mPFC脑区锥体神经元微小兴奋性突触后电流的频率增加(t=4.009,P<0.01),微小抑制性突触后电流的幅度增加(t=2.172,P<0.05)。结论反复禁锢应激能够增强小鼠mPFC脑区锥体神经元的兴奋性和抑制性突触传递。这些mPFC脑区锥体神经元突触传递的可塑性改变可能与反复禁锢应激导致的焦虑相关行为改变密切相关。Objective To investigate the effect of repeated restraint stress on synaptic activity of pyramidal neurons in the medial prefrontal cortex(mPFC)of wild-type C57BL6/J mice.Methods Male C57BL6/J mice,aged 12-20 weeks,were randomly divided into control group and restraint group,and the mice in the restraint group were given repeated restraint stress,1 hour per day for 4 consecutive days.The elevated plus maze test was used to evaluate the anxiety-like behaviors of mice,and whole-cell patch clamp technique was used to record the excitatory and inhibitory postsynaptic currents of pyramidal neurons in the mPFC.Results The mice in the restraint group had a significant increase in anxiety-like behaviors(t=3.069,P<0.01),as well as significant increases in the frequency of miniature excitatory postsynaptic currents(t=4.009,P<0.01)and the amplitude of miniature inhibitory postsynaptic currentsin pyramidal neurons of the mPFC(t=2.172,P<0.05).Conclusion Repeated restraint stress can enhance the excitatory and inhibitory synaptic transmission in pyramidal neurons of the mPFC,and such plasticity changes in synaptic transmission of pyramidal neurons in the mPFC may be closely associated with the changes in anxiety-related behaviors caused by repeated restraint stress.

关 键 词：应激 生理学 额叶前皮质 神经元 突触传递 小鼠 

分 类 号：R338.2[医药卫生—人体生理学]