雨生红球藻虾青素调控下珍珠龙胆石斑鱼抗氧化能力及相关基因的加权基因共表达网络分析  

Analysis of antioxidant capacity and weighted gene co-expression network of related genes in grouper(Epinephelus lanceolatus♂×E.fuscoguttatus♀)under the regulation of Haematococcus pluvialis astaxanthin

作　　者：高金伟 姜智飞 张文慧 周文礼 卢文玉[1] GAO Jinwei;JIANG Zhifei;ZHANG Wenhui;ZHOU Wenli;LU Wenyu(School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China;Tianjin Key Laboratory of Aquaecology and Aquaculture,College of Fisheries,Tianjin Agricultural University,Tianjin 300392,China;Tianjin Agricultural Ecological Environment Monitoring and Agricultural Product Quality Testing Center,Tianjin 300193,China)

机构地区：[1]天津大学化工学院,天津300072 [2]天津市水产生态及养殖重点实验室,天津农学院水产学院,天津300392 [3]天津市农业生态环境监测与农产品质量监测中心,天津300193

出　　处：《海洋渔业》2024年第5期586-598,共13页Marine Fisheries

基　　金：稻蟹藻循环生态种养技术集成与示范(22ZXBTSN00020);天津市科技计划项目。

摘　　要：为研究雨生红球藻(Haematococcus pluvialis)虾青素对珍珠龙胆石斑鱼(Epinephelus lanceolatus♂×E.fuscoguttatus♀)生长及抗氧化能力的影响,制备了虾青素分别为0 mg·kg^(-1)(对照组)、48.56 mg·kg^(-1)(低剂量组)和189.56 mg·kg^(-1)(高剂量组)的3种饲料,开展了为期60 d的饲养实验。分析了石斑鱼生长性能和血清抗氧化能力指标,并联合差异表达基因(DEGs)和加权基因共表达网络(WGCNA)分析,鉴定了抗氧化相关的共表达基因模块,根据模块内基因的连接度筛选核心基因。结果显示,低剂量组和高剂量组相比对照组,石斑鱼末体质量(FBW)、增重率(WGR)和特定生长率(SGR)均显著增加(P<0.05),超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-PX)活力显著升高(P<0.05),丙二醛(MDA)含量显著降低(P<0.05);低剂量组和高剂量组分别筛选出DEGs 3089个和3012个,DEGs主要富集到Jak-STAT信号通路、趋化因子信号通路和AMPK信号通路。WGCNA分析共构建了17个共表达模块,筛选得到black、turquoise、magenta和lightgreen共4个具有生物学意义且与抗氧化显著相关(相关系数的绝对值>0.60)的特异性共表达模块,GO和KEGG功能富集分析均表明,特异性模块富集到的Jak-STAT信号通路和Autophagy信号通路在珍珠龙胆石斑鱼抗氧化过程中具有重要的作用。选取连通性最高的前20个基因进行核心基因筛选,通过基因功能注释最终筛选到14个核心基因。以核心基因为节点构建的共表达网络中包括zf-C2H2、zf-BED和zf-MIZ等转录因子。结果表明,雨生红球藻虾青素可以提高珍珠龙胆石斑鱼的生长性能和抗氧化能力,筛选得到的核心基因可能在珍珠龙胆石斑鱼的抗氧化调控中发挥重要作用,研究结果可为石斑鱼优质饲料的开发提供参考。In order to study the effects of Haematococcus pluvialis astaxanthin on the growth and antioxidant capacity of E.lanceolatus♂×E.fuscoguttatus♀,three types of feeds containing astaxanthin 0 mg·kg^(-1)(control group),48.56 mg·kg^(-1)(lowdose group)and 189.56 mg·kg^(-1)(high-dose group)were prepared.The growth performance and serum biochemical parameters of the grouper were examined after a 60-day feeding experiment.The antioxidant related co-expression gene modules were idenified by combination of differentially expression genes(DEGs)and WGCNA.The hub genes were identified according to the connectivity of genes in the modules.The results showed that the final body weight(FBW),weight gain rate(WGR),specific growth rate(SGR),and the activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-PX)were significantly higher in experimented groups than those in the control group(P<0.05),while the content of malondialdehyde(MDA)was significantly lower in experimented groups(P<0.05).A total of 3089 and 3012 DEGs were identified in the low and high dose groups,respectively.These DEGs were mainly enriched in Jak-STAT,chemokine,and AMPK signaling pathway.Co-expression analysis identified 17 modules,in which four(black,turquoise,magenta,and light green)were significantly associated with antioxidants.KEGG functional enrichment analyses were performed based on these four specific modules,which could be enriched in Jak-STAT signaling pathway and autophagy pathway.The top 20 hub genes were selected by calculating the gene connectivity in the corresponding networks,and 14 hub genes were finally screened by functional annotation.Transcription factors such as zf-C2H2、zf-BED and zf-MIZ were also included in the co-expression network.In sum,Haematococcus pluvialis astaxanthin can improve the growth performance and antioxidant capacity of E.lanceolatus♂×E.fuscoguttatus♀.The hub genes may be crucial in the control of antioxidants,which provides references for the development of more effective grouper fe

关 键 词：转录组分析 珍珠龙胆石斑鱼 抗氧化 加权基因共表达网络分析 

分 类 号：S917.4[农业科学—水产科学]