2018-2020年南昌市生活污水中非脊灰肠道病毒监测分析  

Surveillance for non-poliovirus enteroviruses from domestic sewage in Nanchang from 2018 to 2020

作　　者：肖芳[1] 刘晓庆[2] 李健雄[2] 施勇[2] 周珺[2] 刘师文[2] 徐刚[2] 张艳妮[2] 肖大瑾 冉鑫 Xiao Fang;Liu Xiaoqing;Li Jianxiong;Shi Yong;Zhou Jun;Liu Shiwen;Xu Gang;Zhang Yanni;Xiao Dajin;Ran Xin(Young Scientific Research and Innovation Team,Jiangxi Provincial Center for Disease Control and Prevention,Jiangxi Provincial Key Laboratory of Major Epidemic Prevention and Control(Grant NO.2024SSY06021),Nanchang 330029,China;Institute for Disease Control and Inspection,Jiangxi Provincial Center for Disease Control and Prevention,Nanchang 330029,China)

机构地区：[1]江西省疾病预防控制中心青年科研创新攻关团队、重大疫情防控江西省重点实验室,南昌330029 [2]江西省疾病预防控制中心疾病控制检验所,南昌330029

出　　处：《国际病毒学杂志》2024年第5期413-416,共4页International Journal of Virology

基　　金：江西省卫生健康委科技计划(20204848)。

摘　　要：目的 了解南昌市生活污水中非脊灰肠道病毒型别.方法 从2018年1月至2020年12月,在南昌市某污水处理厂每月采集生活污水样本,用RD和Hep-2细胞对阴离子膜吸附—碱洗脱法富集的病毒进行分离.通过反转录-聚合酶链式反应(RT-PCR)对收获的毒株核酸进行扩增,并对获得的VP1区扩增片段进行序列分析.结果 2018-2020年共采集生活污水36份,通过RD、Hep-2两种细胞共分离非脊灰肠道病毒(non-polio enterovirus,NPEV)207株,包含8种不同血清型,均属于HEV-B组.南昌市生活污水中常见非脊灰肠道病毒的优势基因型为ECHO 11、CoxB3、ECHO 6和ECHO 7,且ECHO 11、ECHO 6和ECHO 7在环境污水和AFP病例中存在共循环现象.结论 2018-2020年南昌市生活污水NPEV检出率较高,需持续开展外环境NPEV监测以评估NPEV相关疾病的风险.Objective To understand the types of non-poliovirus enteroviruses(NPEV)from domestic sewage in Nanchang city.Methods The domestic sewage samples were collected from a sewage processing plant in Nanchang during January 2018 to December 2020.RD and Hep-2 cell culture were used in virus isolation for samples enriched by the anionic membrane adsorption-alkali washing method.The reverse transcription polymerase chain reaction(RT-PCR)was used to amplify nucleic acid from harvested virus strains and the amplified VP1 region were used for sequence analysis.Results A total of 36 domestic sewage samples were collected from 2018 to 2020,and 207 NPEV strains were isolated from RD and Hep-2 cells.The strains belonged to 8 serotypes in the HEV-B group.The common dominant genotypes were ECHO 11,CoxB 3,ECHO 6 and ECHO 7 in the domestic sewage of Nanchang city while ECHO 11,ECHO 6 and ECHO 7 were co-circulating in environmental sewage and AFP cases.Conclusions The detection rate of NPEV in domestic sewage in Nanchang was high from 2018 to 2020.Continuous environmental surveillance for NPEV would be necessary to assess the risk of NPEV-related diseases.

关 键 词：生活污水 非脊灰肠道病毒 环境监测 

分 类 号：R123.1[医药卫生—环境卫生学]