高脂饲料对斑马鱼脂代谢及KLF15-TWIST2信号通路表达的影响  

Effect of a high-fat diet on lipid metabolism and KLF15-TWIST2 signaling pathway gene expression levels in zebrafish(Danio rerio)

作　　者：杜雨凌 李虹辉 王家辉 彭丽雯 朱豪杰 全珂 罗庆华 褚武英[2] DU Yuling;LI Honghui;WANG Jiahui;PENG Liwen;ZHU Haojie;QUAN Ke;LUO Qinghua;CHU Wuying(School of Food Science and Bioengineering,Changsha University of Science&Technology,Changsha 410114,China;College of Biological and Chemical Engineering,Changsha University,Hunan Engineering Technology Research Center for Amphibian and Reptile Resource Protection and Product Processing,Changsha 410022,China)

机构地区：[1]长沙理工大学食品与生物工程学院,湖南长沙410114 [2]长沙学院生物与化学工程学院,湖南省两栖爬行动物资源保护与产品加工工程技术研究中心,湖南长沙410022

出　　处：《中国水产科学》2024年第9期1079-1089,共11页Journal of Fishery Sciences of China

基　　金：国家自然科学基金青年基金项目(32102816);湖南省教育厅科学研究优秀青年项目(22B0327);国家自然科学基金区域创新发展联合基金项目(U21A20263);国家重点研发计划项目(2022YFD2400802).

摘　　要：为探究KLF15-TWIST2信号通路在斑马鱼(Danio rerio)脂代谢中的作用,分析了高脂饲料对斑马鱼生理指标、组织形态和抗氧化酶活性的影响,并探讨了脂代谢相关基因及KLF15-TWIST2信号通路的表达特征。结果显示,斑马鱼摄入高脂饲料引起总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)含量显著上升(P<0.01),且体内出现大量脂滴;超氧化物歧化酶(SOD)活性显著上升(P<0.05),谷胱甘肽过氧化物酶(GSH-PX)活性显著降低(P<0.001)。此外,摄入高脂饲料显著增加了KLF15基因的表达水平(P<0.001),而TWIST2表达显著降低(P<0.05);脂肪合成相关基因SREBP、FASN、ACC1、DGAT2、CEBPα表达显著升高(P<0.05),脂肪分解基因ATGL显著降低(P<0.05),而脂肪酸氧化基因PPARα、CPT1、LPL、LXPα则表现出显著升高趋势(P<0.05)。相关性分析表明KLF15-TWIST2信号通路与SREBP、FASN、ACC1、ATGL、DGAT2、CEBPα、PPARα、LPL等基因的表达都具有相关性。综上所述,高脂饲料能够引起斑马鱼体内过量脂质蓄积并导致脂代谢紊乱,推测KLF15-TWIST2信号通路可能对脂代谢具有重要调控作用。Lipids are important constituents and energy storage substances in fish,and their main role is oxidative energy supply.Excessive lipid levels in the feed affect the normal physiological metabolism of fish.KLF15 is an important regulator and effector of intra-and inter-organ metabolic processes,playing a key role in promoting fat synthesis and storage.TWIST2 is a conserved basic helix-loop-helix(b-HLH)transcription factor that serves as a molecular switch to activate or repress target genes through direct or indirect mechanisms,regulating pathways involved in inflammation,energy homeostasis,and metabolic disorders.The expression patterns of KLF15 with TWIST2 play important roles in many aquatic animals.This study aimed to investigate the role of the KLF15-TWIS2 signaling pathway in lipid metabolism in zebrafish(Danio rerio).Zebrafish larvae were randomly divided into a normal-fat diet(NFD)group and a high-fat diet(HFD)group 5 days after fertilization.The NFD group was fed a basal diet and the HFD group was fed an HFD.After 7 consecutive days of feeding,zebrafish litters from the NFD and HFD groups were homogenized in an ice bath to collect samples,centrifuged to extract the supernatant and measured to analyse changes in lipid metabolism-related indexes and the expression characteristics of the KLF15-TWIST2 signaling pathway.Physiological indices and antioxidant enzyme activities as well as histological observations were used to determine whether an HFD caused lipid metabolism disorders.The expression levels of KLF15,TWIST2,and genes related to lipid metabolism(SREBP,FASN,ACC1,DGAT2,CEBPα,CPT1,ATGL,LPL,SCD,and HSL)were detected by fluorescence quantitative PCR and their correlations were analyzed.An HFD caused a significant(P<0.01)increase in total cholesterol,triglyceride,and low-density lipoprotein-cholesterol content in zebrafish.A significant(P<0.05)increase in superoxide dismutase activity and a significant(P<0.001)decrease in glutathione peroxidase activity were observed in zebrafish in the HFD group compare

关 键 词：斑马鱼 KLF15-TWIST2信号通路 高脂饲料 脂代谢 

分 类 号：S963[农业科学—水产养殖]