不同增殖速度的东海原甲藻细胞周期及转录组比较分析  

Comparative analysis of the cell cycle progression and transcriptome of Prorocentrum donghaiense with different proliferation rates

作　　者：赵鹏珍 王云龙[2] 马欣云 全为民[2] 李楠楠 陈渊戈[2] 范瑞良 张海燕[2] 徐清 欧阳珑玲 ZHAO Pengzhen;WANG Yunlong;MA Xinyun;QUAN Weimin;LI Nannan;CHEN Yuange;FAN Ruiliang;ZHANG Haiyan;XU Qing;OUYANG Longling(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of East China Sea Fishery Resources Exploitation,Ministry of Agriculture and Rural Affairs,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China;Ningbo Marine Center,Ministry of Natural Resources,Ningbo 315012,China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国水产科学研究院东海水产研究所,农业农村部东海渔业资源开发利用重点实验室,上海200090 [3]自然资源部宁波海洋中心,浙江宁波315012

出　　处：《中国水产科学》2024年第9期1116-1128,共13页Journal of Fishery Sciences of China

基　　金：中国水产科学研究院东海水产研究所基本科研业务费项目(2016T04).

摘　　要：为研究不同增殖速度的东海原甲藻(Prorocentrum donghaiense)细胞周期时相分布特点以及内在调控机理,利用流式细胞检测技术比较了两种不同增殖速度的东海原甲藻细胞周期时相差异,并利用RNA-seq技术对两组细胞转录模式进行了比较分析。结果显示,相比增殖速度较快的实验组,增殖速度较慢的实验组S期更短,且部分细胞阻滞在G2/M期。因此,S期的持续时间和G2/M期转换是否被阻滞可以决定东海原甲藻的增殖速率。两组细胞差异表达基因富集程度最大且富集最显著的GO分类均与微管和细胞骨架有关,暗示两实验组细胞周期进程的差异由细胞中的微管动态变化不同造成。注释到49个细胞周期蛋白编码基因、74个细胞周期蛋白依赖性激酶编码基因和26个细胞分裂周期蛋白编码基因。其中,1个CYCB和2个CDK1基因与调节东海原甲藻G2/M期阻滞有关,1个CYCA、1个CYCU、4个CDK2和1个Cdc48基因与藻细胞S期进程调控有关。Prorocentrum donghaiense is one of the main red tide organisms in China’s coastal waters.It is characterized by frequent outbreaks,a large area of influence,and serious harm.The cell cycle is an important biological process that regulates cell division.It can be affected by environmental factors,resulting in changes in the growth rate of phytoplankton.Therefore,it is valuable to investigate the differences in the cell cycle progression of P.donghaiense with different proliferation rates to help understanding its proliferation characteristics.The cell cycle pattern of P.donghaiense has been preliminary explored.Genes and proteins related to the cell cycle have been isolated and identified using omics approaches.However,there is a lack of research on the characteristics of the cell cycle with different proliferation rates,as well as the molecular mechanism.Our previous studies demonstrated that the growth rate of P.donghaiense varies under different environmental conditions.To compare the cell cycle progression of P.donghaiense with different proliferation rates and understand its molecular mechanism,we selected two experimental groups with slower(group 3)and faster(group 16)proliferation rates,and analyzed them using flow cytometry and RNA sequencing technology.The results showed that group 3 cells had a shorter S phase and were partially blocked in G2/M phase.A total of 149219 unigenes were obtained,of which 6081 were annotated in the NR,GO,KEGG,eggNOG,Swiss-Prot,and Pfam databases.A total of 114358 unigenes were classified into three terms,namely,cellular components,molecular functions,and bioprocesses,with 57 categories in the GO database.The 30554 unigenes annotated in the eggNOG database were classified into 25 categories.GO classification and pathway enrichment results of differentially expressed genes(DEGs)between the two experimental groups showed that the most important DEGs were related to microtubules and the cytoskeleton.We hypothesized that the differences in cell cycle progression between the two e

关 键 词：东海原甲藻 增殖速度 细胞周期时相 转录组 差异表达基因 赤潮 

分 类 号：S931[农业科学—渔业资源]