高效液相色谱法同时测定除痰止嗽丸中白花前胡甲素、白花前胡乙素和橙皮苷  

Simultaneous determination of Praeruptorin A,Praeruptorin B and Hesperidin in Chutan Zhisou Pills by high performance liquid chromatography

作　　者：曹蓉蓉 张卫莲 CAO Rongrong;ZHANG Weiian(Yulin Institute for Drug Control,Yulin 719000,China)

机构地区：[1]榆林市药品检验所,陕西榆林719000

出　　处：《化学分析计量》2024年第11期74-78,106,共6页Chemical Analysis And Meterage

基　　金：榆林市青年人才托举项目(20210349)。

摘　　要：建立了高效液相色谱法同时测定除痰止嗽丸中白花前胡甲素、白花前胡乙素和橙皮苷的含量。采用Agilent ZORBAX SB-C18色谱柱(250 mm×4.6 mm,5μm)分离,以乙腈-0.2%(体积分数)磷酸溶液作为流动相进行梯度洗脱,流量为1.0mL/min,检测波长分别为321nm(白花前胡甲素、白花前胡乙素)、284nm(橙皮苷),柱温为30℃,进样体积为5μL。白花前胡甲素、白花前胡乙素和橙皮苷3种成分的质量在各自范围内与色谱峰面积线性关系良好,相关系数均不小于0.9995。样品的平均回收率为96.90%~102.98%,测定结果的相对标准偏差为0.44%~0.90%(n=6)。该方法稳定性强、准确度高,可用于除痰止嗽丸的质量控制。A high performance liquid chromatography method was established for the simultaneous determination of Prohemin A,Prohemin B and Hesperidin in Chutanzhisou Pills.The separation was carried out on Agilent ZORBAXSB-C18 column(250 mm×4.6 mm,5μm),and gradient elution was performed with acetonitrile-0.2%(volume fraction)phosphoric acid solution as mobile phase with a flow rate of 1.0 mL/min.The detection wavelength was 321 nm(Prohemin A,Prohemin B)and 284 nm(Hesperidin),the column temperature was 30℃,and the sample volume was 5μL.The quality of the three components were linearly correlated with the chromatographic peak area in their respective ranges,and the correlation coefficients were no less than 0.9995.The average recoveries were 96.90%-102.98%,and the relative standard deviation was 0.44%-0.90%(n=6).The method has strong stability and high accuracy,and can be used for quality control of Chutanzhisou Pills.

关 键 词：高效液相色谱法 除痰止嗽丸 白花前胡甲素 白花前胡乙素 橙皮苷 

分 类 号：O657.7[理学—分析化学]