四溴双酚A对秀丽隐杆线虫寿命的影响及作用机制的研究  

Lifespan and underlying mechanism of tetrabromobisphenol A in Caenorhabditis elegans

作　　者：刘馥雯 胡晓钧 LIU Fuwen;HU Xiaojun(School of Chemical and Environmental Engineering,Shanghai Institute of Technology,Shanghai 201418,China)

机构地区：[1]上海应用技术大学化学与环境工程学院,上海201418

出　　处：《应用技术学报》2024年第4期395-401,439,共8页Journal of Technology

基　　金：上海市科技创新行动计划扬帆专项(23YF1445900);上海市曙光计划项目(17SG52);辽宁省自然科学基金(2019-ZD-0550);上海市高校青年教师培养资助计划项目(ZZ202212053);上海应用技术大学引进人才基金项目(YJ2021-28)资助。

摘　　要：四溴双酚A(tetrabromobisphenol A,TBBPA)是一种应用广泛的新型溴系阻燃剂,其是否存在生态环境风险的问题,引起了全球广泛的关注。选用秀丽隐杆线虫作为受试生物,探究TBBPA在近环境浓度(1~200μg·L^(–1))下对线虫自L1期亚急性暴露72 h,线虫的寿命、超氧化物歧化酶、过氧化氢酶、丙二醛含量及胁迫相关基因表达的影响。结果表明,与对照组相比,当TBBPA暴露浓度大于等于10μg·L–1时线虫的平均寿命发生显著性抑制。随着TBBPA暴露浓度的增加线虫体内的超氧化物歧化酶、过氧化氢酶及丙二醛的累积程度逐渐增强。胁迫相关基因的表达最为敏感,经突变体验证所得ctl-2基因参与调控线虫的寿命。结合皮尔逊相关性分析结果,TBBPA诱导产生自由基对线虫机体造成的伤害可作用于其寿命,超氧化物歧化酶及过氧化氢酶参与了TBBPA诱导毒性效应过程中的解毒机制。Tetrabromobisphenol A(TBBPA)is a widely used new brominated flame retardant,the ecological risk of TBBPA received increasing attention.In current study,the nematode Caenorhabditis elegans(C.elegans)was selected to investigate the toxic effects of TBBPA at environmentally realistic concentrations(1~200μg·L–1)from L1 larvae after 72 h subacute exposure,the endpoints including longevity,superoxide dismutase enzyme,catalase enzyme,malondialdehyde content and stress-related gene expressions were measured.The results showed that the average lifespan of C.elegans was significantly inhibited at TBBPA exposure concentrations greater than or equal to 10μg·L^(-1).The levels of superoxide dismutase enzyme,catalase enzyme and malondialdehyde content in nematode gradually enhanced with the increasing TBBPA exposure concentrations.The stress-related gene expressions showed the highest sensitive.The text of mutant nematodes validated that the ctl-2 gene is involved in determining the lifespan of C.elegans.Pearson correlation analysis indicating that the damage of free radicals to C.elegans induced by TBBPA could further act on its lifespan,while superoxide dismutase enzyme and catalase enzyme worked as a detoxification in the process.

关 键 词：四溴双酚A 秀丽隐杆线虫 毒性效应 寿命 氧化应激 

分 类 号：X327[环境科学与工程—环境工程]